lambdahHEX-15-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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lambdahHEX-15
lambdahHEX-15
规格:
货期:
编号:TS135914
品牌:Testobio
产品名称: lambdahHEX-15
商品货号: TS135914
Designations: lambdahHEX-15
Species: Homo sapiens, human
Applications:
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Vector:
Construct size (kb): 46.59999847412109
Insert:
DNA: cDNA
Insert lengths(kb): 3.599999904632568
Tissue: kidney
Gene product: hexokinase 1 HK1
Alleles: A2, A1
Insert Size (kb): 3.600
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 15.0, 10.0, 3.5; HindIII--24.0, 10.0, 8.4, 4.6, 3.1; BamHI--20.0, 12.0, 7.0, 5.6, 4.8, 3.3, 1.9, 1.4; SalI--38.0, 13.5; XbaI--52.0.
The insert includes nucleotides 38-3598, encoding a complete 917 amino acid protein. There are 43 bp 5 untranslated and 765 bp 3 untranslated sequence in this insert.
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Enzyme(s) not detecting polymorphism: BclI, BglI, BglII, EcoRI, MspI, PstI, RsaI, SphI, TaqI, XbaI in a panel of 14 unrelated individuals.
References:

Nishi S, et al. HincII RFLP at the human hexokinase I (HK1) locus on chromosome 10. Nucleic Acids Res. 17: 7547, 1989. PubMed: 2571978

Nishi S, et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157: 937-943, 1988. PubMed: 3207429

Graeme I Bell, personal communication

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lambdahHEX-15

  • 货号: TS135914
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: lambdahHEX-15
商品货号: TS135914
Designations: lambdahHEX-15
Species: Homo sapiens, human
Applications:
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Vector:
Construct size (kb): 46.59999847412109
Insert:
DNA: cDNA
Insert lengths(kb): 3.599999904632568
Tissue: kidney
Gene product: hexokinase 1 HK1
Alleles: A2, A1
Insert Size (kb): 3.600
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 15.0, 10.0, 3.5; HindIII--24.0, 10.0, 8.4, 4.6, 3.1; BamHI--20.0, 12.0, 7.0, 5.6, 4.8, 3.3, 1.9, 1.4; SalI--38.0, 13.5; XbaI--52.0.
The insert includes nucleotides 38-3598, encoding a complete 917 amino acid protein. There are 43 bp 5 untranslated and 765 bp 3 untranslated sequence in this insert.
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Enzyme(s) not detecting polymorphism: BclI, BglI, BglII, EcoRI, MspI, PstI, RsaI, SphI, TaqI, XbaI in a panel of 14 unrelated individuals.
References:

Nishi S, et al. HincII RFLP at the human hexokinase I (HK1) locus on chromosome 10. Nucleic Acids Res. 17: 7547, 1989. PubMed: 2571978

Nishi S, et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157: 937-943, 1988. PubMed: 3207429

Graeme I Bell, personal communication

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