Mfn2-null MEFs-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Mfn2-null MEFs
Mfn2-null MEFs
规格:
货期:
编号:TS136340
品牌:Testobio
产品名称： Mfn2-null MEFs
商品货号： TS136340
Organism： Mus musculus, mouse
Tissue： embryo fibroblast
Cell Type： fibroblast
Product Format： frozen
Morphology： fibroblast-like
Culture Properties： adherent
Biosafety Level： 2 xa0 Cells contain SV40 viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： embryo, 10.5-days gestation
Applications：
These cell lines are useful in studying mitochondrial biology and the role of mitochondrial fusion in cell physiology.
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
MEFs were derived from embryos 10.5 days gestation. Embryos were mechanically dispersed by repeated passage through a P1000 pipette tip and plated with MEF medium (DME, 10% FCS, 1X nonessential amino acids, 1 mM L-glutamine (Invitrogen/Gibco)). Cells with deletions of Mfn1 (ATCC CRL-2992) or Mfn2 (TS136340) or both Mfn1/Mfn2-null MEFs (ATCC CRL-2994) were subsequently immortalized by transduction with SV-40 T antigen.
Clinical Data：
Donor Organism Characteristics: knockout
Comments：
Both Mfn1 and Mfn2 are essential for mitochondrial fusion which is essential for embryonic development.xa0
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing：
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation ratio: A subcultivation ratio of 1:5 to 1:20 is recommended

Medium renewal: Every 2 to 3 days
Cryopreservation：
Freeze medium: complete growth medium, 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor： D Chan
Year of Origin： 2000
References：

Chen H, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160: 189-200, 2003. PubMed: 12527753

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Mfn2-null MEFs

  • 货号: TS136340
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Mfn2-null MEFs
商品货号： TS136340
Organism： Mus musculus, mouse
Tissue： embryo fibroblast
Cell Type： fibroblast
Product Format： frozen
Morphology： fibroblast-like
Culture Properties： adherent
Biosafety Level： 2 xa0 Cells contain SV40 viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age： embryo, 10.5-days gestation
Applications：
These cell lines are useful in studying mitochondrial biology and the role of mitochondrial fusion in cell physiology.
Storage Conditions： liquid nitrogen vapor phase
Images：
Derivation：
MEFs were derived from embryos 10.5 days gestation. Embryos were mechanically dispersed by repeated passage through a P1000 pipette tip and plated with MEF medium (DME, 10% FCS, 1X nonessential amino acids, 1 mM L-glutamine (Invitrogen/Gibco)). Cells with deletions of Mfn1 (ATCC CRL-2992) or Mfn2 (TS136340) or both Mfn1/Mfn2-null MEFs (ATCC CRL-2994) were subsequently immortalized by transduction with SV-40 T antigen.
Clinical Data：
Donor Organism Characteristics: knockout
Comments：
Both Mfn1 and Mfn2 are essential for mitochondrial fusion which is essential for embryonic development.xa0
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing：
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation ratio: A subcultivation ratio of 1:5 to 1:20 is recommended

Medium renewal: Every 2 to 3 days
Cryopreservation：
Freeze medium: complete growth medium, 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor： D Chan
Year of Origin： 2000
References：

Chen H, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160: 189-200, 2003. PubMed: 12527753

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