| 产品名称： | pSYC1174 CMCC 2422 |
|---|---|
| 商品货号： | TS136417 |
| Designations： | pSYC1174 CMCC 2422 |
| GenBank Number： | I02088 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | Chiron Corp. |
| Applications： | produces protein methionine aminopeptidase |
| Vector： | Construct size (kb): 5.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: map Gene name: methionine aminopeptidase Contains complete coding sequence?: Y Strain: CM89 Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 3.2 Cross references: DNA Seq. Acc.: I02088 Insert lengths(kb): 3.200000047683716 Gene product: methionine aminopeptidase map Target Gene: methionine aminopeptidase |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.4, 2.6; PstI--3.7, 2.2; SmaI--5.9. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989 Ben-Bassat A, et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli aminopeptidase and its gene structure. J. Bacteriol. 169: 751-757, 1987. PubMed: 3027045 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,870,017 dated Sep 26 1989 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 5,013,662 dated May 7 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pSYC1174 CMCC 2422 |
|---|---|
| 商品货号： | TS136417 |
| Designations： | pSYC1174 CMCC 2422 |
| GenBank Number： | I02088 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | Chiron Corp. |
| Applications： | produces protein methionine aminopeptidase |
| Vector： | Construct size (kb): 5.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: map Gene name: methionine aminopeptidase Contains complete coding sequence?: Y Strain: CM89 Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 3.2 Cross references: DNA Seq. Acc.: I02088 Insert lengths(kb): 3.200000047683716 Gene product: methionine aminopeptidase map Target Gene: methionine aminopeptidase |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.4, 2.6; PstI--3.7, 2.2; SmaI--5.9. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989 Ben-Bassat A, et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli aminopeptidase and its gene structure. J. Bacteriol. 169: 751-757, 1987. PubMed: 3027045 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,870,017 dated Sep 26 1989 Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 5,013,662 dated May 7 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
