| 产品名称： | EBO-pSV2-neo |
|---|---|
| 商品货号： | TS136931 |
| Designations： | EBO-pSV2-neo |
| Depositors： | RF Margolskee |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.3599996566772500 Vector: EBO-pSV2-neo (plasmid) Promoters: Promoter SV40 late Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph) Marker(s):G418R,ampR,hygR Construct size (kb): 13.35999965667725 Features: marker(s): ampR, hygR, G418R promoter: SV40 late replicon: pMB1, EBV oriP |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA. A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally. The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588 Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549 Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831 Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662 Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915 |
| Shipped： | freeze-dried |
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| 产品名称： | EBO-pSV2-neo |
|---|---|
| 商品货号： | TS136931 |
| Designations： | EBO-pSV2-neo |
| Depositors： | RF Margolskee |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.3599996566772500 Vector: EBO-pSV2-neo (plasmid) Promoters: Promoter SV40 late Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph) Marker(s):G418R,ampR,hygR Construct size (kb): 13.35999965667725 Features: marker(s): ampR, hygR, G418R promoter: SV40 late replicon: pMB1, EBV oriP |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA. A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally. The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588 Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549 Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831 Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662 Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915 |
| Shipped： | freeze-dried |
