| 产品名称： | pBCRF1 |
|---|---|
| 商品货号： | TS137005 |
| Designations： | pBCRF1 |
| GenBank Number： | V01555 |
| Species： | Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors： | DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Vector： | Name of vector: pcDSRalpha Construct size (kb): 4.30 Type of vector: plasmid Construction: pcDV1, HTLV-1 LTR Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): centromere: c marker(s): ampR promoter: SV40/HTLV-1 LTR replicon: pMB1, SV40 |
| Insert： | Genome: Epstein-Barr virus
Gene symbol: BCRF1 Gene name: open reading frame BCRF1 Contains complete coding sequence?: U Type of DNA: genomic Insert size (kb): 0.7 Cross references: DNA Seq. Acc.: V01555DNA: genomic Insert lengths(kb): 0.70
Gene product: open reading frame BCRF1 BCRF1 |
| Insert Size (kb)： | 0.70 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 0.7; EcoRI--3.8, 0.5; XhoI--3.6, 0.7. EcoRI will also excise the insert. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| Classification： | Herpesviridae, Lymphocryptovirus |
| References： | Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510 Hsu DH, et al. Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1. Science 250: 830-832, 1990. PubMed: 2173142 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pBCRF1 |
|---|---|
| 商品货号： | TS137005 |
| Designations： | pBCRF1 |
| GenBank Number： | V01555 |
| Species： | Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors： | DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Vector： | Name of vector: pcDSRalpha Construct size (kb): 4.30 Type of vector: plasmid Construction: pcDV1, HTLV-1 LTR Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): centromere: c marker(s): ampR promoter: SV40/HTLV-1 LTR replicon: pMB1, SV40 |
| Insert： | Genome: Epstein-Barr virus
Gene symbol: BCRF1 Gene name: open reading frame BCRF1 Contains complete coding sequence?: U Type of DNA: genomic Insert size (kb): 0.7 Cross references: DNA Seq. Acc.: V01555DNA: genomic Insert lengths(kb): 0.70
Gene product: open reading frame BCRF1 BCRF1 |
| Insert Size (kb)： | 0.70 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 0.7; EcoRI--3.8, 0.5; XhoI--3.6, 0.7. EcoRI will also excise the insert. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| Classification： | Herpesviridae, Lymphocryptovirus |
| References： | Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510 Hsu DH, et al. Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1. Science 250: 830-832, 1990. PubMed: 2173142 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
