| 产品名称： | pCMV5YFQ1919 |
|---|---|
| 商品货号： | TS137107 |
| Designations： | pCMV5YFQ1919 |
| GenBank Number： | J02933 |
| Species： | Homo sapiens, human |
| Depositors： | W Cheung |
| Applications： | in another host, produces protein coagulation factor VII (serum prothrombin conversion accelerator) |
| Vector： | Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCMV5 Intact vector size: 4.700 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI BamHI HindIII Polylinker sites: Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): promoter: CMV, -> terminator: hGH, -> replicon: SV40 replicon: pMB1 marker(s): ampR, replicon: f1, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: F7 Genomic copy number: unique Gene name: coagulation factor VII (serum prothrombin conversion accelerator) Contains complete coding sequence?: U Chromosome: 13; Localization: 13 q34 Genome: Homo sapiens Gene symbol: F9 Genomic copy number: unique Gene name: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) Contains complete coding sequence?: U Chromosome: X; Localization: X q26.3-q27.1 Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 2.7 Cross references: DNA Seq. Acc.: J02933 Insert lengths(kb): 2.700000047683716 Gene product: coagulation factor VII (serum prothrombin conversion accelerator) F7 Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor VII (serum prothrombin conversion accelerator) |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.7, 2.7; EcoRI--5.0, 2.5; EcoRV--7.4; PstI--4.7, 2.7; SacI--6.8, 0.5. Digestion with EcoRI produces fragments of 5.0 and 2.4 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes a chimera in which aa 1-48 of processed human factor VII (Gla domain), with mutations A1Y, L8F, R9V, P10Q, K32E, D33N, A34T, K38T, L39E, replace the corresponding region of factor IX. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370 Toomey JR, et al. Localization of the human tissue factor recognition determinant of human factor VIIa. J. Biol. Chem. 266: 19198-19202, 1991. PubMed: 1918037 Wing-Fai Cheung, personal communication |
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| 产品名称： | pCMV5YFQ1919 |
|---|---|
| 商品货号： | TS137107 |
| Designations： | pCMV5YFQ1919 |
| GenBank Number： | J02933 |
| Species： | Homo sapiens, human |
| Depositors： | W Cheung |
| Applications： | in another host, produces protein coagulation factor VII (serum prothrombin conversion accelerator) |
| Vector： | Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCMV5 Intact vector size: 4.700 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI BamHI HindIII Polylinker sites: Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): promoter: CMV, -> terminator: hGH, -> replicon: SV40 replicon: pMB1 marker(s): ampR, replicon: f1, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: F7 Genomic copy number: unique Gene name: coagulation factor VII (serum prothrombin conversion accelerator) Contains complete coding sequence?: U Chromosome: 13; Localization: 13 q34 Genome: Homo sapiens Gene symbol: F9 Genomic copy number: unique Gene name: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) Contains complete coding sequence?: U Chromosome: X; Localization: X q26.3-q27.1 Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 2.7 Cross references: DNA Seq. Acc.: J02933 Insert lengths(kb): 2.700000047683716 Gene product: coagulation factor VII (serum prothrombin conversion accelerator) F7 Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor VII (serum prothrombin conversion accelerator) |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.7, 2.7; EcoRI--5.0, 2.5; EcoRV--7.4; PstI--4.7, 2.7; SacI--6.8, 0.5. Digestion with EcoRI produces fragments of 5.0 and 2.4 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes a chimera in which aa 1-48 of processed human factor VII (Gla domain), with mutations A1Y, L8F, R9V, P10Q, K32E, D33N, A34T, K38T, L39E, replace the corresponding region of factor IX. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370 Toomey JR, et al. Localization of the human tissue factor recognition determinant of human factor VIIa. J. Biol. Chem. 266: 19198-19202, 1991. PubMed: 1918037 Wing-Fai Cheung, personal communication |
