| 产品名称： | pKG1800 |
|---|---|
| 商品货号： | TS137180 |
| Designations： | pKG1800 |
| Depositors： | M Rosenberg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.9000000953674320 Vector: pKG1800 (plasmid) Construction: pKO1 Marker(s):ampR Construct size (kb): 4.900000095367432 Features: insert detection: galK marker(s): ampR replicon: pMB1 |
| Applications： | terminator-cloning vector |
| Comments： | Insertion of terminator sequences at the cloning sites causes the galK+ phenotype to become galK-. galK+ plasmids are lethal on galETK hosts because of galactose-1-phosphate accumulation, so terminator-containing clones survive in galactose media. A terminator cloning plasmid vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056 Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988 |
| Shipped： | freeze-dried |
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| 产品名称： | pKG1800 |
|---|---|
| 商品货号： | TS137180 |
| Designations： | pKG1800 |
| Depositors： | M Rosenberg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.9000000953674320 Vector: pKG1800 (plasmid) Construction: pKO1 Marker(s):ampR Construct size (kb): 4.900000095367432 Features: insert detection: galK marker(s): ampR replicon: pMB1 |
| Applications： | terminator-cloning vector |
| Comments： | Insertion of terminator sequences at the cloning sites causes the galK+ phenotype to become galK-. galK+ plasmids are lethal on galETK hosts because of galactose-1-phosphate accumulation, so terminator-containing clones survive in galactose media. A terminator cloning plasmid vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056 Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988 |
| Shipped： | freeze-dried |
