| 产品名称： | pKA-1 |
|---|---|
| 商品货号： | TS137551 |
| Designations： | pKA-1 |
| GenBank Number： | U07562 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 19.10000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ABL1 Other gene symbols: ABL Genomic copy number: unique Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1 Contains complete coding sequence?: N Chromosome: 9; Localization: 9 q34.1 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 16.421 Cross references: DNA Seq. Acc.: U07562 Nucleotides 1-16321 of the insert correspond to nucleotides 42692-59012 of U07562. Insert lengths(kb): 16.42099952697754 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 ABL Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1 |
| Insert Size (kb)： | 16.421 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.2, 6.4, 2.3, 2.1; BglI--7.2, 5.5, 2.5, 2.2, 1.3, 1.2; HindIII--11.5, 7.6, 0.82; PstI--5.1, 4.8, 3.75, 2.7, 2.1, 0.73, 0.64; EcoRI--7.0, 5.0, 2.65, 2.15, 1.1, 0.92, 0.38. The insert contains part of the intron between exon 1b and exon 1a. |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |
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| 产品名称： | pKA-1 |
|---|---|
| 商品货号： | TS137551 |
| Designations： | pKA-1 |
| GenBank Number： | U07562 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 19.10000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ABL1 Other gene symbols: ABL Genomic copy number: unique Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1 Contains complete coding sequence?: N Chromosome: 9; Localization: 9 q34.1 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 16.421 Cross references: DNA Seq. Acc.: U07562 Nucleotides 1-16321 of the insert correspond to nucleotides 42692-59012 of U07562. Insert lengths(kb): 16.42099952697754 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 ABL Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1 |
| Insert Size (kb)： | 16.421 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.2, 6.4, 2.3, 2.1; BglI--7.2, 5.5, 2.5, 2.2, 1.3, 1.2; HindIII--11.5, 7.6, 0.82; PstI--5.1, 4.8, 3.75, 2.7, 2.1, 0.73, 0.64; EcoRI--7.0, 5.0, 2.65, 2.15, 1.1, 0.92, 0.38. The insert contains part of the intron between exon 1b and exon 1a. |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |
