| 产品名称： | pHUCI |
|---|---|
| 商品货号： | TS137650 |
| Designations： | pHUCI |
| GenBank Number： | X07884 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: COL1A1 Genomic copy number: unique Gene name: collagen, type I, alpha 1 Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q21.3-q22 Tissue: fibroblast cell line SV80 Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 5.2 Cross references: DNA Seq. Acc.: X07884 Nucleotides 1-1500 of the insert correspond to nucleotides 1-1500 of X07884. Insert lengths(kb): 5.199999809265137 Tissue: fibroblast cell line SV80 Gene product: collagen, type I, alpha 1 COL1A1 |
| Insert Size (kb)： | 5.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.3; EcoRI--4.6, 2.7, 0.64; PvuII--2.5, 2.0, 1.5, 0.65, 0.5, 0.35; SacI--7.0, 0.52; XhoI--4.9, 2.4, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.9; EcoRI--4.5; KpnI--0.2; NcoI--0.6, 0.7; PvuII--0.6, 0.85, 2.3, 4.3; SacI--1.0, 1.5; XhoI--0.8, 1.5. To obtain a high yield, grow the culture in minimal media with casamino acids (M9), followed by chloramphenicol amplification to increase the plasmid copy number. |
| References： | Tromp G, et al. Structure of a full-length cDNA clone for the prepro alpha1(I) chain of human type I procollagen. Biochem. J. 253: 919-922, 1988. PubMed: 3178743 Helena Kuivaniemi, personal communication |
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| 产品名称： | pHUCI |
|---|---|
| 商品货号： | TS137650 |
| Designations： | pHUCI |
| GenBank Number： | X07884 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: COL1A1 Genomic copy number: unique Gene name: collagen, type I, alpha 1 Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q21.3-q22 Tissue: fibroblast cell line SV80 Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 5.2 Cross references: DNA Seq. Acc.: X07884 Nucleotides 1-1500 of the insert correspond to nucleotides 1-1500 of X07884. Insert lengths(kb): 5.199999809265137 Tissue: fibroblast cell line SV80 Gene product: collagen, type I, alpha 1 COL1A1 |
| Insert Size (kb)： | 5.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.3; EcoRI--4.6, 2.7, 0.64; PvuII--2.5, 2.0, 1.5, 0.65, 0.5, 0.35; SacI--7.0, 0.52; XhoI--4.9, 2.4, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.9; EcoRI--4.5; KpnI--0.2; NcoI--0.6, 0.7; PvuII--0.6, 0.85, 2.3, 4.3; SacI--1.0, 1.5; XhoI--0.8, 1.5. To obtain a high yield, grow the culture in minimal media with casamino acids (M9), followed by chloramphenicol amplification to increase the plasmid copy number. |
| References： | Tromp G, et al. Structure of a full-length cDNA clone for the prepro alpha1(I) chain of human type I procollagen. Biochem. J. 253: 919-922, 1988. PubMed: 3178743 Helena Kuivaniemi, personal communication |
