| 产品名称： | Hepatitis A virus |
|---|---|
| 商品货号： | TS137937 |
| Classification： | Picornaviridae, Hepatovirus |
| Agent： | Hepatitis A virus |
| Strain： | HM175/18f |
| Applications： | Efficacy testing Viruscide testing Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Strain demonstrates a rapid replication/cytopathic effect (RR/CPE+) phenotype in BS-C-1 cells, but retains antigenic characteristics of low cell culture passage hepatitis A virus. CPE more pronounced in FRhK4 cells. |
| Effect on Host： | CPE, refractile rounding and degeneration |
| Recommended Host： | FRhK4 (ATCC® CRL-1688™) |
| Growth Conditions： | Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa06-7 days
|
| Name of Depositor： | SM Lemon |
| Source： | Human fecal material passaged in marmosets. Virus isolated from marmoset liver in primary cells then passaged in BS-C-1 cells. |
| References： | Lemon SM, et al. Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. J. Virol. 65: 2056-2065, 1991. PubMed: 1705995 Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
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| 产品名称： | Hepatitis A virus |
|---|---|
| 商品货号： | TS137937 |
| Classification： | Picornaviridae, Hepatovirus |
| Agent： | Hepatitis A virus |
| Strain： | HM175/18f |
| Applications： | Efficacy testing Viruscide testing Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Strain demonstrates a rapid replication/cytopathic effect (RR/CPE+) phenotype in BS-C-1 cells, but retains antigenic characteristics of low cell culture passage hepatitis A virus. CPE more pronounced in FRhK4 cells. |
| Effect on Host： | CPE, refractile rounding and degeneration |
| Recommended Host： | FRhK4 (ATCC® CRL-1688™) |
| Growth Conditions： | Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa06-7 days
|
| Name of Depositor： | SM Lemon |
| Source： | Human fecal material passaged in marmosets. Virus isolated from marmoset liver in primary cells then passaged in BS-C-1 cells. |
| References： | Lemon SM, et al. Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. J. Virol. 65: 2056-2065, 1991. PubMed: 1705995 Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
