HFBCL85-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HFBCL85
HFBCL85
规格:
货期:
编号:TS138035
品牌:Testobio
产品名称: HFBCL85
商品货号: TS138035
Designations: HFBCL85
GenBank Number:

M85674

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GAD1
Other gene symbols: GAD
Genomic copy number: unique
Gene name: glutamate decarboxylase 1 (brain)
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q31
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M85674; EST: EST02192; Primer: M13 Forward
Insert lengths(kb): 1.200000047683716
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: glutamate decarboxylase 1 (brain)(glutamic acid decarboxylase) GAD
Insert Size (kb): 1.200
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; PstI--4.2; HindIII--4.2.
EST02192 matches GenBank accession HUMGAD with 100% identity over the sequence range: 227-14.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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HFBCL85

  • 货号: TS138035
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HFBCL85
商品货号: TS138035
Designations: HFBCL85
GenBank Number:

M85674

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GAD1
Other gene symbols: GAD
Genomic copy number: unique
Gene name: glutamate decarboxylase 1 (brain)
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q31
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M85674; EST: EST02192; Primer: M13 Forward
Insert lengths(kb): 1.200000047683716
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: glutamate decarboxylase 1 (brain)(glutamic acid decarboxylase) GAD
Insert Size (kb): 1.200
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; PstI--4.2; HindIII--4.2.
EST02192 matches GenBank accession HUMGAD with 100% identity over the sequence range: 227-14.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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