pRS169
pRS169
规格:
货期:
编号:TS138190
品牌:Testobio
| 产品名称： | pRS169 |
|---|---|
| 商品货号： | TS138190 |
| Designations： | pRS169 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.8000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 5.800 Type of vector: phagemid Cloning sites: XhoI SalI EcoRI SmaI BamHI NotI SacI Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL1 replicon: pMB1, f1, ARS1 Vector: pRS169 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):URA3,ampR Construct size (kb): 5.800000190734863 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL1 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--2.9 (doublet); BamHI--5.8; EcoRI--5.8; EcoRV--4.5, 1.3; HindIII--5.8; SalI--5.8; SmaI--5.8; XbaI--uncut. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL1 promoter (no ATG included in the sequence) proceeds through the polylinker. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the URA3 gene was inserted into the NdeI site and a DraIII/EcoRI fragment containing the GAL1 and GAL10 promoters was inserted into the Asp718 site of the MCS of pRSS56. All ends were blunted. The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL1 promoter - GAL10 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pRS169 |
|---|---|
| 商品货号： | TS138190 |
| Designations： | pRS169 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.8000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 5.800 Type of vector: phagemid Cloning sites: XhoI SalI EcoRI SmaI BamHI NotI SacI Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL1 replicon: pMB1, f1, ARS1 Vector: pRS169 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):URA3,ampR Construct size (kb): 5.800000190734863 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL1 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--2.9 (doublet); BamHI--5.8; EcoRI--5.8; EcoRV--4.5, 1.3; HindIII--5.8; SalI--5.8; SmaI--5.8; XbaI--uncut. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL1 promoter (no ATG included in the sequence) proceeds through the polylinker. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the URA3 gene was inserted into the NdeI site and a DraIII/EcoRI fragment containing the GAL1 and GAL10 promoters was inserted into the Asp718 site of the MCS of pRSS56. All ends were blunted. The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL1 promoter - GAL10 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
