sNF02.2

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货期:

编号:TS138204

品牌:Testobio

产品名称：	sNF02.2
商品货号：	TS138204
Organism：	Homo sapiens, human
Tissue：	derived from metastatic site: lung
Cell Type：	Schwann Cell
Product Format：	frozen
Morphology：	Schwann cell-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neurofibromatosis type 1 (NF1)
Age：	35 year old
Gender：	male
Storage Conditions：	Liquid nitrogen vapor phase
Images：
Derivation：	The sNF02.2 was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin protein.
Clinical Data：	35 years male
Comments：	Western immunoblotting showed apparently full-length neurofibromin protein.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm². flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 10³ to 8 X 10³ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when cell concentration is between 3 X 10⁴ and 4 X 10⁴ cells/cm². Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 twice weekly is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Culture medium,90%; DMSO,10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: 5% CO₂ in air recommended Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 8,12 vWA: 16
Population Doubling Time：	about 26 hours
Name of Depositor：	DF Muir, MR Wallace
Deposited As：	Homo sapiens
Passage History：	xadThe cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. xad
Year of Origin：	2002
References：	Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937 Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442

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sNF02.2

货号: TS138204
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产品名称：	sNF02.2
商品货号：	TS138204
Organism：	Homo sapiens, human
Tissue：	derived from metastatic site: lung
Cell Type：	Schwann Cell
Product Format：	frozen
Morphology：	Schwann cell-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neurofibromatosis type 1 (NF1)
Age：	35 year old
Gender：	male
Storage Conditions：	Liquid nitrogen vapor phase
Images：
Derivation：	The sNF02.2 was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin protein.
Clinical Data：	35 years male
Comments：	Western immunoblotting showed apparently full-length neurofibromin protein.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm². flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 10³ to 8 X 10³ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when cell concentration is between 3 X 10⁴ and 4 X 10⁴ cells/cm². Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 twice weekly is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Culture medium,90%; DMSO,10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: 5% CO₂ in air recommended Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 8,12 vWA: 16
Population Doubling Time：	about 26 hours
Name of Depositor：	DF Muir, MR Wallace
Deposited As：	Homo sapiens
Passage History：	xadThe cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. xad
Year of Origin：	2002
References：	Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937 Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442