U-CH2

规格:

货期:

编号:TS138299

品牌:Testobio

产品名称：	U-CH2
商品货号：	TS138299
Organism：	Homo sapiens, human
Tissue：	sacral bone
Product Format：	frozen
Morphology：	Mesencymal like, with variable vacuoles
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor, chordoma
Age：	72 years
Gender：	female
Ethnicity：	Caucasian
Applications：	Use as a modelxa0for chordoma which is a rare slow-growing tumor The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Shipping Information：	frozen
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	This cell line was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma.
Genes Expressed：	amplification of transcription factor T (brachyury)
Comments：	U-CH2 is a human chordoma cell line that was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma. It exhibits chordoma-like characteristics, and has molecular, genetic, and morphological features typical of chordoma.xa0 Chordoma is a rare slow-growing tumor type, and U-CH2 is a relatively slow-growing cell line.xa0 U-CH2 has a heterogeneous morphology consisting of physaliferous cells with mucinous intercellular substance, which represent typical chordoma features. The cells contain amplification of transcription factor T (Brachyury) that is most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a nonprofit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for chordoma.
Complete Growth Medium：	Iscoves Modified Dulbeccos Medium (IMDM; ATCC^® No. 30-2005): RPMI-1640 Medium (ATCC^® No. 30-2001) (4:1) + 10% FBS (ATCC^® No. 30-2020) + additional 1% L-glutamine (ATCC^® No. 30-2214)
Subculturing：	Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50 μg/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions. Volumes used in this subculture protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 5.0ml Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in 10ml fresh growth medium. Add appropriate aliquots of the cell suspension to new coated culture vessels. Incubate cultures at 37°C.
Cryopreservation：	Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions：	Temperatue: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0TH01: 9.3 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D5S818: 10, 11xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D13S317: 11 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D7S820: 8, 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D16S539: 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 CSF1PO: 11, 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Amelogenin: X xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 vWA: 17 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 TPOX: 8
Passage Number：	20
Name of Depositor：	Silke Bruderlein, Peter Moller
Year of Origin：	September 11, 2000
References：	Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487 Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078

首页 > 产品中心 > 微生物培养 > 菌株 > null > U-CH2

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

U-CH2

货号: TS138299
好评

有货

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	U-CH2
商品货号：	TS138299
Organism：	Homo sapiens, human
Tissue：	sacral bone
Product Format：	frozen
Morphology：	Mesencymal like, with variable vacuoles
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor, chordoma
Age：	72 years
Gender：	female
Ethnicity：	Caucasian
Applications：	Use as a modelxa0for chordoma which is a rare slow-growing tumor The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Shipping Information：	frozen
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	This cell line was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma.
Genes Expressed：	amplification of transcription factor T (brachyury)
Comments：	U-CH2 is a human chordoma cell line that was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma. It exhibits chordoma-like characteristics, and has molecular, genetic, and morphological features typical of chordoma.xa0 Chordoma is a rare slow-growing tumor type, and U-CH2 is a relatively slow-growing cell line.xa0 U-CH2 has a heterogeneous morphology consisting of physaliferous cells with mucinous intercellular substance, which represent typical chordoma features. The cells contain amplification of transcription factor T (Brachyury) that is most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a nonprofit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for chordoma.
Complete Growth Medium：	Iscoves Modified Dulbeccos Medium (IMDM; ATCC^® No. 30-2005): RPMI-1640 Medium (ATCC^® No. 30-2001) (4:1) + 10% FBS (ATCC^® No. 30-2020) + additional 1% L-glutamine (ATCC^® No. 30-2214)
Subculturing：	Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50 μg/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions. Volumes used in this subculture protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 5.0ml Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in 10ml fresh growth medium. Add appropriate aliquots of the cell suspension to new coated culture vessels. Incubate cultures at 37°C.
Cryopreservation：	Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions：	Temperatue: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0TH01: 9.3 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D5S818: 10, 11xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D13S317: 11 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D7S820: 8, 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 D16S539: 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 CSF1PO: 11, 12 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Amelogenin: X xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 vWA: 17 xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 TPOX: 8
Passage Number：	20
Name of Depositor：	Silke Bruderlein, Peter Moller
Year of Origin：	September 11, 2000
References：	Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487 Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078