| 产品名称： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
|---|---|
| 商品货号： | TS138385 |
| Classification： | Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic |
| Agent： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
| Strain： | 4070A envelope strain |
| Applications： | Analytical methodologies Pharmaceutical and Personal Care |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448) |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | This virus produces plaques on PG-4 (feline S+L-) cells (ATCC CRL-2032), and foci of rounded cells on MiCL1 (Mink S+L-) cells (ATCC CCL-64.1). This holding is prepared by growing ATCC VR-1448 and harvesting the supernate. At the time of deposit, cell-free preparations of the Mo/A-MuLV strain were considered as suitable reference standards in assays to detect replication competent murine retrovirus--select lots have had titer confirmed by workers outside the ATCC. amphotropic |
| Effect on Host： | No, in vitro effects: Cytopathic effects in NIH3T3 cells No CPE in NIH3T3 cells |
| Recommended Host： | NIH/3T3 (tk-) (ATCC CRL-1658) Recommended Host: tk(-) NIH3T3 (ATCC® CRL-1658x99) many mammalian cell lines (mouse, rat, human) |
| Growth Conditions： | Duration: 5-7 days at 37.0°C in NIH/3T3 (tk-) (ATCC CRL-1658) |
| Cryopreservation： | Storage temperature: -70C |
| Name of Depositor： | CA Wilson |
| Special Collection： | NCRR Contract |
| Source： | Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448) |
| References： | Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952 General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 |
| Cross References： | Nucleotide (GenBank) : AF010170 Plasmid pAMS with hybrid amphotropic/Moloney murine leukemia virus, complete sequence. |
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| 产品名称： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
|---|---|
| 商品货号： | TS138385 |
| Classification： | Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic |
| Agent： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
| Strain： | 4070A envelope strain |
| Applications： | Analytical methodologies Pharmaceutical and Personal Care |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448) |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | This virus produces plaques on PG-4 (feline S+L-) cells (ATCC CRL-2032), and foci of rounded cells on MiCL1 (Mink S+L-) cells (ATCC CCL-64.1). This holding is prepared by growing ATCC VR-1448 and harvesting the supernate. At the time of deposit, cell-free preparations of the Mo/A-MuLV strain were considered as suitable reference standards in assays to detect replication competent murine retrovirus--select lots have had titer confirmed by workers outside the ATCC. amphotropic |
| Effect on Host： | No, in vitro effects: Cytopathic effects in NIH3T3 cells No CPE in NIH3T3 cells |
| Recommended Host： | NIH/3T3 (tk-) (ATCC CRL-1658) Recommended Host: tk(-) NIH3T3 (ATCC® CRL-1658x99) many mammalian cell lines (mouse, rat, human) |
| Growth Conditions： | Duration: 5-7 days at 37.0°C in NIH/3T3 (tk-) (ATCC CRL-1658) |
| Cryopreservation： | Storage temperature: -70C |
| Name of Depositor： | CA Wilson |
| Special Collection： | NCRR Contract |
| Source： | Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448) |
| References： | Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952 General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 |
| Cross References： | Nucleotide (GenBank) : AF010170 Plasmid pAMS with hybrid amphotropic/Moloney murine leukemia virus, complete sequence. |
