| 产品名称： | Myf-5/18-7 |
|---|---|
| 商品货号： | TS138397 |
| Designations： | Myf-5/18-7 |
| Species： | Homo sapiens, human |
| Applications： | The 3 XhoII/EcoRI fragment has been used as a specific probe. A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library. |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.259999990463257 Tissue: fetal skeletal muscle Gene product: myogenic factor 5 MYF5 |
| Insert Size (kb)： | 1.260 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp)(approx): EcoRI/PstI- 50- HaeII- 100- HaeII- 15- HincII- 70- XhoII- 15- FokI/PstI- 265- XhoII- 35- HinfI- 400- HinfI/PvuII- 310- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.2; HincII--4.2, 0.26; PvuII--2.7, 1.3, 0.36; SphI--3.8, 0.41; HindIII--4.5. This is a shorter version of Myf-5 which extends from the PstI site at nucleotide 185 to 1430. It does not include the complete coding sequence, which extends from nucleotide 43 through 807. Shows weak cross-hybridization to the mouse MyoD1 probe. Amino acid sequence has a region highly similar to myc proteins and the Drosophila developmental factors achaete scute and twist. The 3 XhoII/EcoRI fragment has been used as a specific probe. A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library. |
| References： | Braun T, et al. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 8: 701-709, 1989. PubMed: 2721498 Braun T, et al. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J. 8: 3617-3625, 1989. PubMed: 2583111 Miner JH, Wold B. Herculin, a fourth member of the MyoD family of myogenic regulator genes. Proc. Natl. Acad. Sci. USA 87: 1089-1093, 1990. PubMed: 2300571 Hans H Arnold, personal communication |
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| 产品名称： | Myf-5/18-7 |
|---|---|
| 商品货号： | TS138397 |
| Designations： | Myf-5/18-7 |
| Species： | Homo sapiens, human |
| Applications： | The 3 XhoII/EcoRI fragment has been used as a specific probe. A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library. |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.259999990463257 Tissue: fetal skeletal muscle Gene product: myogenic factor 5 MYF5 |
| Insert Size (kb)： | 1.260 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp)(approx): EcoRI/PstI- 50- HaeII- 100- HaeII- 15- HincII- 70- XhoII- 15- FokI/PstI- 265- XhoII- 35- HinfI- 400- HinfI/PvuII- 310- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.2; HincII--4.2, 0.26; PvuII--2.7, 1.3, 0.36; SphI--3.8, 0.41; HindIII--4.5. This is a shorter version of Myf-5 which extends from the PstI site at nucleotide 185 to 1430. It does not include the complete coding sequence, which extends from nucleotide 43 through 807. Shows weak cross-hybridization to the mouse MyoD1 probe. Amino acid sequence has a region highly similar to myc proteins and the Drosophila developmental factors achaete scute and twist. The 3 XhoII/EcoRI fragment has been used as a specific probe. A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library. |
| References： | Braun T, et al. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 8: 701-709, 1989. PubMed: 2721498 Braun T, et al. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J. 8: 3617-3625, 1989. PubMed: 2583111 Miner JH, Wold B. Herculin, a fourth member of the MyoD family of myogenic regulator genes. Proc. Natl. Acad. Sci. USA 87: 1089-1093, 1990. PubMed: 2300571 Hans H Arnold, personal communication |
