HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
规格:
货期:
编号:TS138529
品牌:Testobio
产品名称: HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
商品货号: TS138529
Designations: HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
GenBank Number:

M10060

Species: human T-cell leukemia virus II
Depositors: Irvin S. Y. Chen, Department of Medicine, University of California School of Medicine, Los Angeles, CA
Applications:
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
Vector: Vector name: pBR322
Intact vector size (in kb): 4.363
Type of vector: plasmid
Vector ends: HindIII

Features :
marker(s):
tetR, ampR
replicon: pMB1
DNA Seq. Acc.: J01749
Insert: Genome: human T-cell leukemia virus II
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert ends: HindIII
Insert size (kb): 10.1
DNA Seq. Acc.: M10060
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: 200 ng dried plasmid DNA
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.0 (doublet), 0.92, 0.36; HindIII--10.5, 3.2; XbaI--13.5.
The insert contains the following restriction sites (approximate kb from 5 end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4.
The viral portion of the insert is 8.8kb.
The viral portion of the insert is 8.8kb.
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells.
Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb.
References:

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323

Irvin S Chen, personal communication

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HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA

  • 货号: TS138529
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
商品货号: TS138529
Designations: HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
GenBank Number:

M10060

Species: human T-cell leukemia virus II
Depositors: Irvin S. Y. Chen, Department of Medicine, University of California School of Medicine, Los Angeles, CA
Applications:
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
Vector: Vector name: pBR322
Intact vector size (in kb): 4.363
Type of vector: plasmid
Vector ends: HindIII

Features :
marker(s):
tetR, ampR
replicon: pMB1
DNA Seq. Acc.: J01749
Insert: Genome: human T-cell leukemia virus II
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert ends: HindIII
Insert size (kb): 10.1
DNA Seq. Acc.: M10060
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: 200 ng dried plasmid DNA
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.0 (doublet), 0.92, 0.36; HindIII--10.5, 3.2; XbaI--13.5.
The insert contains the following restriction sites (approximate kb from 5 end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4.
The viral portion of the insert is 8.8kb.
The viral portion of the insert is 8.8kb.
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells.
Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb.
References:

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323

Irvin S Chen, personal communication

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