YEplac112
YEplac112
规格:
货期:
编号:TS138654
品牌:Testobio
| 产品名称： | YEplac112 |
|---|---|
| 商品货号： | TS138654 |
| Designations： | YEplac112 |
| Depositors： | RD Gietz |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.989 Vector: YEplac112 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.989 Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: lac replicon: 2 micron ori replicon: pMB1 MCS: HindIII...EcoRI |
| Applications： | YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | This is a YE-type (yeast episomal) vector with the TRP1 marker. The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106 |
| Related Products： | component of:ATCC 87623 component of:ATCC 87638 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
YEplac112
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | YEplac112 |
|---|---|
| 商品货号： | TS138654 |
| Designations： | YEplac112 |
| Depositors： | RD Gietz |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.989 Vector: YEplac112 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.989 Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: lac replicon: 2 micron ori replicon: pMB1 MCS: HindIII...EcoRI |
| Applications： | YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | This is a YE-type (yeast episomal) vector with the TRP1 marker. The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106 |
| Related Products： | component of:ATCC 87623 component of:ATCC 87638 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |
