lambda7-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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lambda7
lambda7
规格:
货期:
编号:TS138803
品牌:Testobio
产品名称: lambda7
商品货号: TS138803
Designations: lambda7
GenBank Number:

L10641

Species: Homo sapiens, human
Depositors: A Dugaiczyk
Vector:
Construct size (kb): 50.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.1
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19100 of the insert correspond to
nucleotides ~29850-49180 of L10641.
Insert lengths(kb): 19.10000038146973
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC
Insert Size (kb): 19.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 7.2, 4.3, 3.3, 2.7, 1.6; HindIII--10.5, 9.55, 8.8, 5.75, 4.35, 4.1, 1.5, 1.4, 0.9, 0.6, 0.3; BamHI--13, 8, 6.6, 6.2, 3.9 (doublet), 3, 2.3, 1.5, 0.5;
Restriction digests of the clone give the following sizes (kb): BglII--14, 9.2, 5.8, 5, 4.9, 2.7, 2.2, 1.9, 1.8, 1.35, 1, 0.65, 0.35; SstI--18, 11, 4.6, 4.2, 3.85, 2.4, 1.95, 1.65, 1.2.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.6, 4.3, 8.6, 11.9; HindIII--0.4, 1.5, 2.9, 3.8, 4.2, 13.75, 18.1; SacI--14.6, 18.8.
Begins in intron 6 and extends into the 3 flanking region.
ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb.
TS138803/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb.
Contains an Alu repeats, and part of a THE repeat.
References:

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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lambda7

  • 货号: TS138803
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: lambda7
商品货号: TS138803
Designations: lambda7
GenBank Number:

L10641

Species: Homo sapiens, human
Depositors: A Dugaiczyk
Vector:
Construct size (kb): 50.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.1
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19100 of the insert correspond to
nucleotides ~29850-49180 of L10641.
Insert lengths(kb): 19.10000038146973
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC
Insert Size (kb): 19.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 7.2, 4.3, 3.3, 2.7, 1.6; HindIII--10.5, 9.55, 8.8, 5.75, 4.35, 4.1, 1.5, 1.4, 0.9, 0.6, 0.3; BamHI--13, 8, 6.6, 6.2, 3.9 (doublet), 3, 2.3, 1.5, 0.5;
Restriction digests of the clone give the following sizes (kb): BglII--14, 9.2, 5.8, 5, 4.9, 2.7, 2.2, 1.9, 1.8, 1.35, 1, 0.65, 0.35; SstI--18, 11, 4.6, 4.2, 3.85, 2.4, 1.95, 1.65, 1.2.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.6, 4.3, 8.6, 11.9; HindIII--0.4, 1.5, 2.9, 3.8, 4.2, 13.75, 18.1; SacI--14.6, 18.8.
Begins in intron 6 and extends into the 3 flanking region.
ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb.
TS138803/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb.
Contains an Alu repeats, and part of a THE repeat.
References:

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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