Tetraselmis sp.-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Tetraselmis sp.
Tetraselmis sp.
规格:
货期:
编号:TS138824
品牌:Testobio
产品名称： Tetraselmis sp.
商品货号： TS138824
Strain Designations： RG-07 CCMP WTD-3
Application：
Biofuel production
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Seawater sample near Scripps Oceanographic Institute, La Jolla, CA, 1966
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 1747: Tetraselmis medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium, 8.5 mL

Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO in fresh medium.
  4. Mix the cell preparation and the 15% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximatelyxa0-1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 mL of ATCC medium 1747.xa0 Centrifuge at 300 x g for 5 min.
  10. Remove most of the supernatant (=cryoprotectant, which can inhibit growth) and then resuspend the pellet.xa0 Transfer the culture to a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 1747.
  11. Incubate on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 25°C under a 14 hour light (~50 µEinsteins/m2/s irradiance)/10 hour dark cycle.
Name of Depositor： RA Lewin
Chain of Custody：
ATCC
Year of Origin： 1966
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Tetraselmis sp.

  • 货号: TS138824
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Tetraselmis sp.
商品货号： TS138824
Strain Designations： RG-07 CCMP WTD-3
Application：
Biofuel production
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Seawater sample near Scripps Oceanographic Institute, La Jolla, CA, 1966
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 1747: Tetraselmis medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium, 8.5 mL

Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO in fresh medium.
  4. Mix the cell preparation and the 15% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximatelyxa0-1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 mL of ATCC medium 1747.xa0 Centrifuge at 300 x g for 5 min.
  10. Remove most of the supernatant (=cryoprotectant, which can inhibit growth) and then resuspend the pellet.xa0 Transfer the culture to a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 1747.
  11. Incubate on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 25°C under a 14 hour light (~50 µEinsteins/m2/s irradiance)/10 hour dark cycle.
Name of Depositor： RA Lewin
Chain of Custody：
ATCC
Year of Origin： 1966
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