MC-TGS17-51 [MC17-51, Mutatect]

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货期:

编号:TS138876

品牌:Testobio

产品名称：	MC-TGS17-51 MC17-51, Mutatect
商品货号：	TS138876
Organism：	Mus musculus, mouse
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	fibrosarcoma
Gender：	male
Strain：	C57BL/10
Applications：	tumor model
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. The MC1A fibrosarcoma was induced by methylcholanthrene injection into a C57BL/10 mouse. The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. MC1A-C1 cells were treated with N-methyl-N-nitrosourea (MNU) and selected with low concentrations of 6-thioguanine. 6-thioguanine (6-TG) resistant (HPRT-) clones were isolated and screened for tumorigenicity; clone MC-TRG17 was selected. A spontaneously arising 6-thioguanine sensitive, Hypoxanthine - Aminopterin - Thymidine (HAT) resistant revertant named MC-TGS17-51 (TS138876) was identified by selection in HAT. The cells are hypoxanthine phosphoribosyl transferase positive (HPRT+) PubMed 106311342; PubMed: 7577474. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.
Clinical Data：	The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. male
Tumorigenic：	Yes
Effects：	Yes, forms subcutaneous tumors in syngeneic C57BL/6 mice.
Comments：	The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. The MC1A fibrosarcoma was induced by methylcholanthrene injection into a C57BL/10 mouse. The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. MC1A-C1 cells were treated with N-methyl-N-nitrosourea (MNU) and selected with low concentrations of 6-thioguanine. 6-thioguanine (6-TG) resistant (HPRT-) clones were isolated and screened for tumorigenicity; clone MC-TRG17 was selected. A spontaneously arising 6-thioguanine sensitive, Hypoxanthine - Aminopterin - Thymidine (HAT) resistant revertant named MC-TGS17-51 (TS138876) was identified by selection in HAT. The cells are hypoxanthine phosphoribosyl transferase positive (HPRT+) PubMed 106311342; PubMed: 7577474. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 10(3) to 8 X 10(3) viable cells/cm2 is recommended. Incubate cultures at 37°C. Interval: Maintain cultures at a cell concentration between 1 X 10(4) and 2 X 10(5) cells/cm2 Subcultivation Ratio: A subcultivation of 1:4 to 1:8 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Population Doubling Time：	24 hours
Name of Depositor：	HC Birnboim
References：	Birnboim HC, et al. Mutatect: a mouse tumour model for detecting radiation-induced mutations in vivo. Mutat. Res. 430: 275-280, 1999. PubMed: 10631342 Sandhu JK, et al. Effect of dietary vitamin E on spontaneous or nitric oxide donor-induced mutations in a mouse tumor model. J. Natl. Cancer Inst. 92: 1429-1433, 2000. PubMed: 10974079 Haqqani AS, et al. Expression of interleukin-8 promotes neutrophil infiltration and genetic instability in mutatect tumors. Neoplasia Haqqani AS, et al. Constitutive expression of interleukin-8 by Mutatect cells markedly affects their tumor biology. Carcinogenesis 22: 243-250, 2001. PubMed: 11181444 Wilkinson D, et al. Hprt mutants in a transplantable murine tumour arise more frequently in vivo than in vitro. Br. J. Cancer 72: 1234-1240, 1995. PubMed: 7577474

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MC-TGS17-51 [MC17-51, Mutatect]

货号: TS138876
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产品名称：	MC-TGS17-51 MC17-51, Mutatect
商品货号：	TS138876
Organism：	Mus musculus, mouse
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	fibrosarcoma
Gender：	male
Strain：	C57BL/10
Applications：	tumor model
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. The MC1A fibrosarcoma was induced by methylcholanthrene injection into a C57BL/10 mouse. The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. MC1A-C1 cells were treated with N-methyl-N-nitrosourea (MNU) and selected with low concentrations of 6-thioguanine. 6-thioguanine (6-TG) resistant (HPRT-) clones were isolated and screened for tumorigenicity; clone MC-TRG17 was selected. A spontaneously arising 6-thioguanine sensitive, Hypoxanthine - Aminopterin - Thymidine (HAT) resistant revertant named MC-TGS17-51 (TS138876) was identified by selection in HAT. The cells are hypoxanthine phosphoribosyl transferase positive (HPRT+) PubMed 106311342; PubMed: 7577474. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.
Clinical Data：	The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. male
Tumorigenic：	Yes
Effects：	Yes, forms subcutaneous tumors in syngeneic C57BL/6 mice.
Comments：	The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. The MC1A fibrosarcoma was induced by methylcholanthrene injection into a C57BL/10 mouse. The resulting tumor was adapted to in vitro growth and a variant named MC1A-C1 was isolated that was capable of growth both in culture and as subcutaneous tumor in 8 to 9 week old female C57BL/6 mice PubMed 106311342; PubMed: 7577474. MC1A-C1 cells were treated with N-methyl-N-nitrosourea (MNU) and selected with low concentrations of 6-thioguanine. 6-thioguanine (6-TG) resistant (HPRT-) clones were isolated and screened for tumorigenicity; clone MC-TRG17 was selected. A spontaneously arising 6-thioguanine sensitive, Hypoxanthine - Aminopterin - Thymidine (HAT) resistant revertant named MC-TGS17-51 (TS138876) was identified by selection in HAT. The cells are hypoxanthine phosphoribosyl transferase positive (HPRT+) PubMed 106311342; PubMed: 7577474. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 10(3) to 8 X 10(3) viable cells/cm2 is recommended. Incubate cultures at 37°C. Interval: Maintain cultures at a cell concentration between 1 X 10(4) and 2 X 10(5) cells/cm2 Subcultivation Ratio: A subcultivation of 1:4 to 1:8 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Population Doubling Time：	24 hours
Name of Depositor：	HC Birnboim
References：	Birnboim HC, et al. Mutatect: a mouse tumour model for detecting radiation-induced mutations in vivo. Mutat. Res. 430: 275-280, 1999. PubMed: 10631342 Sandhu JK, et al. Effect of dietary vitamin E on spontaneous or nitric oxide donor-induced mutations in a mouse tumor model. J. Natl. Cancer Inst. 92: 1429-1433, 2000. PubMed: 10974079 Haqqani AS, et al. Expression of interleukin-8 promotes neutrophil infiltration and genetic instability in mutatect tumors. Neoplasia Haqqani AS, et al. Constitutive expression of interleukin-8 by Mutatect cells markedly affects their tumor biology. Carcinogenesis 22: 243-250, 2001. PubMed: 11181444 Wilkinson D, et al. Hprt mutants in a transplantable murine tumour arise more frequently in vivo than in vitro. Br. J. Cancer 72: 1234-1240, 1995. PubMed: 7577474