5B1-4-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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5B1-4
5B1-4
规格:
货期:
编号:TS139076
品牌:Testobio
产品名称: 5B1-4
商品货号: TS139076
Designations: 5B1-4
GenBank Number:

L10338

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN1B
Gene name: sodium channel, voltage-gated, type I, beta polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: frontal cortex
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.404
Cross references: DNA Seq. Acc.: L10338
Nucleotides 1-1404 of the insert correspond to
nucleotides 1-1404 of L10338 with 100% identity.
Insert lengths(kb): 1.404000043869019
Tissue: frontal cortex
Gene product: sodium channel, voltage-gated, type I, beta polypeptide(sodium channel, voltage-gated, beta 1 polypeptide) SCN1B
Insert Size (kb): 1.404
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3, 0.72 (doublet); EcoRI--2.9, 1.5; PstI--3.9, 0.45; PvuII--2.5, 1.25, 0.62; SstI--4.4.
The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.28, 0.95; NdeI--0.61; NcoI--0.10; PstI--0.40; PvuII--0.33; SmaI--0.06.
Detects a 1.5 kb transcript in several brain regions and skeletal muscle.
References:

McClatchey AI, et al. The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain. Hum. Mol. Genet. 2: 745-749, 1993. PubMed: 8394762

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5B1-4

  • 货号: TS139076
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: 5B1-4
商品货号: TS139076
Designations: 5B1-4
GenBank Number:

L10338

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN1B
Gene name: sodium channel, voltage-gated, type I, beta polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: frontal cortex
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.404
Cross references: DNA Seq. Acc.: L10338
Nucleotides 1-1404 of the insert correspond to
nucleotides 1-1404 of L10338 with 100% identity.
Insert lengths(kb): 1.404000043869019
Tissue: frontal cortex
Gene product: sodium channel, voltage-gated, type I, beta polypeptide(sodium channel, voltage-gated, beta 1 polypeptide) SCN1B
Insert Size (kb): 1.404
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3, 0.72 (doublet); EcoRI--2.9, 1.5; PstI--3.9, 0.45; PvuII--2.5, 1.25, 0.62; SstI--4.4.
The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.28, 0.95; NdeI--0.61; NcoI--0.10; PstI--0.40; PvuII--0.33; SmaI--0.06.
Detects a 1.5 kb transcript in several brain regions and skeletal muscle.
References:

McClatchey AI, et al. The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain. Hum. Mol. Genet. 2: 745-749, 1993. PubMed: 8394762

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