pHAL [p3964Full]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: 0574-87917803
pHAL [p3964Full]
pHAL [p3964Full]
规格:
货期:
编号:TS139435
品牌:Testobio
产品名称: pHAL p3964Full
商品货号: TS139435
Designations: pHAL p3964Full
GenBank Number:

J05653

Species: Rattus norvegicus, rat
Depositors: RR McInnes
Vector:
Construct size (kb): 5.138000011444092
Insert:
DNA: cDNA
Insert lengths(kb): 2.178999900817871
Tissue: liver
Gene product: histidine ammonia-lyase(histidase) HAL
Insert Size (kb): 2.179
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.24; XhoI--3.9, 0.96, 0.24; SmaI--4.7, 0.48; PvuII--2.5 (doublet).
The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.05; SmaI--0.45; EcoRV--1.0; XhoI--1.02, 1.98; NcoI--1.6; EcoRI--1.95.
Contains the complete coding sequence, with 8 nt of the 5 untranslated region and the complete 3 untranslated region.
The 1.95 kb EcoRI fragment detects RNA of 2.5 kb in rat liver and rat skin (major) and 7.3 and 4.4 (minor).
The 5 end of the insert is adjacent to the T7 promoter of the vector.
References:

Taylor RG, et al. Cloning and expression of rat histidase. Homology to two bacterial histidases and four phenylalanine ammonia-lyases. J. Biol. Chem. 265: 18192-18199, 1990. PubMed: 2120224

Taylor RG, et al. Localization of histidase to human chromosome region 12q22----q24.1 and mouse chromosome region 10C2----D1. Cytogenet. Cell Genet. 56: 178-181, 1991. PubMed: 2055114

Roderick R McInnes, personal communication

首页 > 产品中心 > 微生物培养 > 菌株 > null > pHAL [p3964Full]

pHAL [p3964Full]

  • 货号: TS139435
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pHAL p3964Full
商品货号: TS139435
Designations: pHAL p3964Full
GenBank Number:

J05653

Species: Rattus norvegicus, rat
Depositors: RR McInnes
Vector:
Construct size (kb): 5.138000011444092
Insert:
DNA: cDNA
Insert lengths(kb): 2.178999900817871
Tissue: liver
Gene product: histidine ammonia-lyase(histidase) HAL
Insert Size (kb): 2.179
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.24; XhoI--3.9, 0.96, 0.24; SmaI--4.7, 0.48; PvuII--2.5 (doublet).
The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.05; SmaI--0.45; EcoRV--1.0; XhoI--1.02, 1.98; NcoI--1.6; EcoRI--1.95.
Contains the complete coding sequence, with 8 nt of the 5 untranslated region and the complete 3 untranslated region.
The 1.95 kb EcoRI fragment detects RNA of 2.5 kb in rat liver and rat skin (major) and 7.3 and 4.4 (minor).
The 5 end of the insert is adjacent to the T7 promoter of the vector.
References:

Taylor RG, et al. Cloning and expression of rat histidase. Homology to two bacterial histidases and four phenylalanine ammonia-lyases. J. Biol. Chem. 265: 18192-18199, 1990. PubMed: 2120224

Taylor RG, et al. Localization of histidase to human chromosome region 12q22----q24.1 and mouse chromosome region 10C2----D1. Cytogenet. Cell Genet. 56: 178-181, 1991. PubMed: 2055114

Roderick R McInnes, personal communication

合作单位: