| 产品名称： | Tetrahymena pyriformis (Ehrenberg) Lwoff |
|---|---|
| 商品货号： | TS139484 |
| Strain Designations： | FL 19l |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | pond, Arrowhead Campsite, Rt. 90, East of Mariana, FL, 1987 |
| Product Format： | test tube |
| Type Strain： | no |
| Comments： | amicronucleate Designated as Tetrahymena pyriformis A Differs by 1 bp in the variable 23S rRNA domain from typical Tetrahymena pyriformis strains and by 2 bp from T. pyriformis B strains |
| Medium： | ATCC® Medium 357: Tetrahymena medium |
| Growth Conditions： | Max Temperature: 27.0°C Min Temperature: 15.0°C Duration: axenic |
| Cryopreservation： | CRYOPRESERVATION: Alternative Thawing Procedure xa01.xa0 Aseptically add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.xa0 Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule. 2.xa0xa0 Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant. xa0The cell suspension will pool at the edge of the plate. 3.xa0xa0 Continue to double the volume of the cell suspension at 10 minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v). xa0When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C.xa0 4.xa0xa0 On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask. xa0Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of the cell suspension. xa0Incubate the culture at 25°C. 5.xa0xa0 After culture has been established subculture into fresh xa0xa0xa0xa0xa0 normal medium without sucrose.xa0 |
| Name of Depositor： | DL Nanney, EM Simon |
| Year of Origin： | 1987 |
| References： | Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037 |
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| 产品名称： | Tetrahymena pyriformis (Ehrenberg) Lwoff |
|---|---|
| 商品货号： | TS139484 |
| Strain Designations： | FL 19l |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | pond, Arrowhead Campsite, Rt. 90, East of Mariana, FL, 1987 |
| Product Format： | test tube |
| Type Strain： | no |
| Comments： | amicronucleate Designated as Tetrahymena pyriformis A Differs by 1 bp in the variable 23S rRNA domain from typical Tetrahymena pyriformis strains and by 2 bp from T. pyriformis B strains |
| Medium： | ATCC® Medium 357: Tetrahymena medium |
| Growth Conditions： | Max Temperature: 27.0°C Min Temperature: 15.0°C Duration: axenic |
| Cryopreservation： | CRYOPRESERVATION: Alternative Thawing Procedure xa01.xa0 Aseptically add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.xa0 Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule. 2.xa0xa0 Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant. xa0The cell suspension will pool at the edge of the plate. 3.xa0xa0 Continue to double the volume of the cell suspension at 10 minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v). xa0When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C.xa0 4.xa0xa0 On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask. xa0Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of the cell suspension. xa0Incubate the culture at 25°C. 5.xa0xa0 After culture has been established subculture into fresh xa0xa0xa0xa0xa0 normal medium without sucrose.xa0 |
| Name of Depositor： | DL Nanney, EM Simon |
| Year of Origin： | 1987 |
| References： | Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037 |
