| 产品名称： | pH2.3 |
|---|---|
| 商品货号： | TS139499 |
| Designations： | pH2.3 |
| GenBank Number： | M11717 |
| Species： | Homo sapiens, human |
| Depositors： | RI Morimoto |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: heat shock 70 kDa protein(heat shock protein, 70 kDa) HSPA* Alleles: A1, A2, , A1, A1, A2, A2, A2, A1 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.3, 2.3; HindIII--5.7; EcoRI--5.7; PvuII--uncut; PstI--3.5, 2.2. This probe has been shown to contain the entire coding region for a heat-shock protein of 70 kDa and hybridizes to a polyadenlyated message of 2.6 kb. Includes approximately 60 bp 5 of initiation ATG through approximately 280 bp 3 of termination TAG (including AATAAA). Genomic sequence is transcribed as an uninterrupted transcript of 2.44 kb with an ORF of 1.986 kb. Enzyme(s) not detecting polymorphism (in a panel of 5): HincII, TaqI, MspI. |
| References： | Zlotogora J, et al. Krabbe disease locus mapped to chromosome 14 by genetic linkage. Am. J. Hum. Genet. 47: 37-44, 1990. PubMed: 1971996 Hunt C, Morimoto RI. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc. Natl. Acad. Sci. USA 82: 6455-6459, 1985. PubMed: 3931075 Wu B, et al. Structure and expression of the human gene encoding major heat shock protein HSP70. Mol. Cell. Biol. 5: 330-341, 1985. PubMed: 2858050 Harrison GS, et al. Chromosomal location of human genes encoding major heat-shock protein HSP70. Somatic Cell Mol. Genet. 13: 119-130, 1987. PubMed: 3470951 Barbara Weiffenbach, personal communication |
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| 产品名称： | pH2.3 |
|---|---|
| 商品货号： | TS139499 |
| Designations： | pH2.3 |
| GenBank Number： | M11717 |
| Species： | Homo sapiens, human |
| Depositors： | RI Morimoto |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: heat shock 70 kDa protein(heat shock protein, 70 kDa) HSPA* Alleles: A1, A2, , A1, A1, A2, A2, A2, A1 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.3, 2.3; HindIII--5.7; EcoRI--5.7; PvuII--uncut; PstI--3.5, 2.2. This probe has been shown to contain the entire coding region for a heat-shock protein of 70 kDa and hybridizes to a polyadenlyated message of 2.6 kb. Includes approximately 60 bp 5 of initiation ATG through approximately 280 bp 3 of termination TAG (including AATAAA). Genomic sequence is transcribed as an uninterrupted transcript of 2.44 kb with an ORF of 1.986 kb. Enzyme(s) not detecting polymorphism (in a panel of 5): HincII, TaqI, MspI. |
| References： | Zlotogora J, et al. Krabbe disease locus mapped to chromosome 14 by genetic linkage. Am. J. Hum. Genet. 47: 37-44, 1990. PubMed: 1971996 Hunt C, Morimoto RI. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc. Natl. Acad. Sci. USA 82: 6455-6459, 1985. PubMed: 3931075 Wu B, et al. Structure and expression of the human gene encoding major heat shock protein HSP70. Mol. Cell. Biol. 5: 330-341, 1985. PubMed: 2858050 Harrison GS, et al. Chromosomal location of human genes encoding major heat-shock protein HSP70. Somatic Cell Mol. Genet. 13: 119-130, 1987. PubMed: 3470951 Barbara Weiffenbach, personal communication |
