| 产品名称： | pGFLEX plasmid in E. coli |
|---|---|
| 商品货号： | TS139680 |
| Designations： | pGFLEX plasmid in E. coli |
| Depositors： | WE Fahl, H Manoharan |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.143
Intact vector size: 6.143 Features (with orientation and position when available): |
| Applications： | Encodes removable tag for protein isolation Expression vector Shuttle vector Vector permitting construction of fusion proteins Vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--6.2; XhoI--3.7, 2.0,0.3; SalI--2.4, 2.1, 1.6.
xa0 The pGFLEX expression system allows production of target proteins fused to either the N or C terminus of the GST pi protein and provides rapid purification of target proteins as either GST fusions or native proteins after cleavage with thrombin. High level of expression in E. coli, cos and other mammalian cultured cell lines.
Recommendation for verification: StuI--4.84, 1.30; SalI--2.4, 2.18, 1.56; PstI--6.14; NotI--6.14. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081 Herbert Manoharan, personal communication |
| Related Products： | ATCC 33694 |
| Shipped： | frozen |
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| 产品名称： | pGFLEX plasmid in E. coli |
|---|---|
| 商品货号： | TS139680 |
| Designations： | pGFLEX plasmid in E. coli |
| Depositors： | WE Fahl, H Manoharan |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.143
Intact vector size: 6.143 Features (with orientation and position when available): |
| Applications： | Encodes removable tag for protein isolation Expression vector Shuttle vector Vector permitting construction of fusion proteins Vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--6.2; XhoI--3.7, 2.0,0.3; SalI--2.4, 2.1, 1.6.
xa0 The pGFLEX expression system allows production of target proteins fused to either the N or C terminus of the GST pi protein and provides rapid purification of target proteins as either GST fusions or native proteins after cleavage with thrombin. High level of expression in E. coli, cos and other mammalian cultured cell lines.
Recommendation for verification: StuI--4.84, 1.30; SalI--2.4, 2.18, 1.56; PstI--6.14; NotI--6.14. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081 Herbert Manoharan, personal communication |
| Related Products： | ATCC 33694 |
| Shipped： | frozen |
