| 产品名称： | pGK51 pGSK51 |
|---|---|
| 商品货号： | TS139841 |
| Designations： | pGK51 pGSK51 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | University of Texas Health Science Center, YM Kupersztoch, University of Texas Health Science Center |
| Applications： | produces protein heat-labile enterotoxin produces protein heat-stable enterotoxin |
| Vector： | Construct size (kb): 3.596999883651733 |
| Insert： | DNA: genomic Insert lengths(kb): 0.9110000133514404 Gene product: heat-stable enterotoxin lt Target Gene: heat-labile enterotoxin, heat-stable enterotoxin |
| Insert Size (kb)： | 0.911 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.686, 0.911; EcoRI/HindIII/SstI--2.686, 0.505, 0.406. Sonicated extracts of E. coli HB101 transformed with this construct express both ST and LTB immunoreactivity. The 0.406 kb EcoRI/SstI fragment encodes the heat stable enterotoxin, and the 0.505 kb SstI/HindIII fragment encodes the heat labile enterotoxin. The latter fragment, derived from EWD299 (ATCC 37218), includes the coding region for the B subunit and 3 transcription and translation stop signals, but lacks the ribosome binding site and sequences encoding the signal peptide. The former fragment is at the 5 end and includes the complete coding sequence and 5 regulatory regions (transcription initiation and ribosome binding site) but not the 3 transcription and translation stop signals. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169 Guzman-Verduzco LM, Kupersztoch YM. Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine. Infect. Immun. 57: 645-648, 1989. PubMed: 2643580 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pGK51 pGSK51 |
|---|---|
| 商品货号： | TS139841 |
| Designations： | pGK51 pGSK51 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | University of Texas Health Science Center, YM Kupersztoch, University of Texas Health Science Center |
| Applications： | produces protein heat-labile enterotoxin produces protein heat-stable enterotoxin |
| Vector： | Construct size (kb): 3.596999883651733 |
| Insert： | DNA: genomic Insert lengths(kb): 0.9110000133514404 Gene product: heat-stable enterotoxin lt Target Gene: heat-labile enterotoxin, heat-stable enterotoxin |
| Insert Size (kb)： | 0.911 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.686, 0.911; EcoRI/HindIII/SstI--2.686, 0.505, 0.406. Sonicated extracts of E. coli HB101 transformed with this construct express both ST and LTB immunoreactivity. The 0.406 kb EcoRI/SstI fragment encodes the heat stable enterotoxin, and the 0.505 kb SstI/HindIII fragment encodes the heat labile enterotoxin. The latter fragment, derived from EWD299 (ATCC 37218), includes the coding region for the B subunit and 3 transcription and translation stop signals, but lacks the ribosome binding site and sequences encoding the signal peptide. The former fragment is at the 5 end and includes the complete coding sequence and 5 regulatory regions (transcription initiation and ribosome binding site) but not the 3 transcription and translation stop signals. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169 Guzman-Verduzco LM, Kupersztoch YM. Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine. Infect. Immun. 57: 645-648, 1989. PubMed: 2643580 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
