p2UGCAT
p2UGCAT
规格:
货期:
编号:TS140335
品牌:Testobio
| 产品名称： | p2UGCAT |
|---|---|
| 商品货号： | TS140335 |
| Designations： | p2UGCAT |
| Depositors： | KR Yamamoto |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.5999999046325680 Vector: p2UGCAT (plasmid) Promoters: Promoter CYC1 Construction: p2UG, CAT Marker(s):URA3,ampR Construct size (kb): 7.599999904632568 Features: marker(s): ampR, URA3 promoter: CYC1 replicon: pMB1, 2 micron enhancer: GRE |
| Applications： | YE-type (episomal) shuttle vector expression vector reporter construct shuttle vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6. The URA3 marker is from S. cerevisiae but complements the ura4 allele from Sc. pombe. Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae and Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814). Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene. A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3 to this insert. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906 |
| Shipped： | freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
p2UGCAT
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | p2UGCAT |
|---|---|
| 商品货号： | TS140335 |
| Designations： | p2UGCAT |
| Depositors： | KR Yamamoto |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.5999999046325680 Vector: p2UGCAT (plasmid) Promoters: Promoter CYC1 Construction: p2UG, CAT Marker(s):URA3,ampR Construct size (kb): 7.599999904632568 Features: marker(s): ampR, URA3 promoter: CYC1 replicon: pMB1, 2 micron enhancer: GRE |
| Applications： | YE-type (episomal) shuttle vector expression vector reporter construct shuttle vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6. The URA3 marker is from S. cerevisiae but complements the ura4 allele from Sc. pombe. Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae and Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814). Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene. A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3 to this insert. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906 |
| Shipped： | freeze-dried |
