| 产品名称： | pUB110cop1 |
|---|---|
| 商品货号： | TS140351 |
| Designations： | pUB110cop1 |
| Depositors： | H Leonhardt |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5000000000000000 Vector: pUB110cop1 (plasmid) Promoters: Promoter none Construction: pUB110, pEB114cop1 Marker(s):kanR,neoR Construct size (kb): 4.5 Features: marker(s): kanR, neoR promoter: none replicon: pUB110 |
| Applications： | shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5. The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability. Low copy number shuttle vector. Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130. |
| Media： | ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061 Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622 Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616 |
| Shipped： | freeze-dried |
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| 产品名称： | pUB110cop1 |
|---|---|
| 商品货号： | TS140351 |
| Designations： | pUB110cop1 |
| Depositors： | H Leonhardt |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5000000000000000 Vector: pUB110cop1 (plasmid) Promoters: Promoter none Construction: pUB110, pEB114cop1 Marker(s):kanR,neoR Construct size (kb): 4.5 Features: marker(s): kanR, neoR promoter: none replicon: pUB110 |
| Applications： | shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5. The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability. Low copy number shuttle vector. Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130. |
| Media： | ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061 Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622 Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616 |
| Shipped： | freeze-dried |
