HIBBS11-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HIBBS11
HIBBS11
规格:
货期:
编号:TS140432
品牌:Testobio
产品名称: HIBBS11
商品货号: TS140432
Designations: HIBBS11
GenBank Number:

T09304

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CHP*
Gene name: chaperonin HumAP (Cct, TCP1-related)
Contains complete coding sequence?: N
Sequence homology: Homo sapiens t complex-related protein-1, related sequence
3 (matricin)
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: NotI
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: L20509
Nucleotides 1-364 of the insert correspond to
nucleotides 709-1072 of L20509 with 90% identity.
DNA Seq. Acc.: T09304; EST: EST07197; Primer: M13 Reverse;
THC id: THC123995
DNA Seq. Acc.: X74801
Nucleotides 1-364 of the insert correspond to
nucleotides 626-989 of X74801 with 98% identity.
Insert lengths(kb): 1.299999952316284
Tissue: infant brain, 3 months old (from Bento Soares)
Gene product: chaperonin HumAP (Cct, TCP1-related) CHP*
Insert Size (kb): 1.300
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.3; EcoRI--3.6, 1.1.
Based on the size of the insert and the alignment with GenBank X74801 (1901 nt, CDS 1-1635), this clone appears to contain part of the coding region.
The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII.
References:

Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585

Mark D Adams, personal communication

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HIBBS11

  • 货号: TS140432
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HIBBS11
商品货号: TS140432
Designations: HIBBS11
GenBank Number:

T09304

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CHP*
Gene name: chaperonin HumAP (Cct, TCP1-related)
Contains complete coding sequence?: N
Sequence homology: Homo sapiens t complex-related protein-1, related sequence
3 (matricin)
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: NotI
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: L20509
Nucleotides 1-364 of the insert correspond to
nucleotides 709-1072 of L20509 with 90% identity.
DNA Seq. Acc.: T09304; EST: EST07197; Primer: M13 Reverse;
THC id: THC123995
DNA Seq. Acc.: X74801
Nucleotides 1-364 of the insert correspond to
nucleotides 626-989 of X74801 with 98% identity.
Insert lengths(kb): 1.299999952316284
Tissue: infant brain, 3 months old (from Bento Soares)
Gene product: chaperonin HumAP (Cct, TCP1-related) CHP*
Insert Size (kb): 1.300
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.3; EcoRI--3.6, 1.1.
Based on the size of the insert and the alignment with GenBank X74801 (1901 nt, CDS 1-1635), this clone appears to contain part of the coding region.
The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII.
References:

Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585

Mark D Adams, personal communication

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