| 产品名称： | pAS1-CDK2 |
|---|---|
| 商品货号： | TS140870 |
| Designations： | pAS1-CDK2 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein cyclin-dependent kinase 2 |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAS1 Intact vector size: 7.100 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 DNA-binding domain, -> epitope tag: hemagglutinin (HA), -> restriction site: EcoRI, ->, 4315 MCS: NdeI...SalI, ->, 4395-4422 terminator: ADC1 replicon: pMB1 marker(s): ampR replicon: 2 micron marker(s): TRP1, -> replicon: f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDK2 Gene name: cyclin-dependent kinase 2 Contains complete coding sequence?: U Localization: 12 q13 Type of DNA: cDNA Insert end: Insert end: Insert size (kb): Cross references: Gene product: cyclin-dependent kinase 2 CDK2 Target Gene: cyclin-dependent kinase 2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--7.2, 0.9; EcoRI--8.2; BamHI--8.2; HindIII--4.6, 2.0, 0.9, 0.7; BamHI/HindIII--4.6, 1.8, 0.9, 0.7, 0.2. Used as a negative control in the 2-hybrid cloning system. |
| References： | Elledge SJ, et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911, 1992. PubMed: 1372993 Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 |
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| 产品名称： | pAS1-CDK2 |
|---|---|
| 商品货号： | TS140870 |
| Designations： | pAS1-CDK2 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein cyclin-dependent kinase 2 |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pAS1 Intact vector size: 7.100 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 DNA-binding domain, -> epitope tag: hemagglutinin (HA), -> restriction site: EcoRI, ->, 4315 MCS: NdeI...SalI, ->, 4395-4422 terminator: ADC1 replicon: pMB1 marker(s): ampR replicon: 2 micron marker(s): TRP1, -> replicon: f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDK2 Gene name: cyclin-dependent kinase 2 Contains complete coding sequence?: U Localization: 12 q13 Type of DNA: cDNA Insert end: Insert end: Insert size (kb): Cross references: Gene product: cyclin-dependent kinase 2 CDK2 Target Gene: cyclin-dependent kinase 2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--7.2, 0.9; EcoRI--8.2; BamHI--8.2; HindIII--4.6, 2.0, 0.9, 0.7; BamHI/HindIII--4.6, 1.8, 0.9, 0.7, 0.2. Used as a negative control in the 2-hybrid cloning system. |
| References： | Elledge SJ, et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911, 1992. PubMed: 1372993 Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 |
