| 产品名称： | pAL-A3 |
|---|---|
| 商品货号： | TS140998 |
| Designations： | pAL-A3 |
| Species： | Unidentified bacterium |
| Depositors： | BJ Brown |
| Applications： | produces protein alginate lyase, D-mannuronan specific |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.099999904632568 Gene product: alginate lyase, D-mannuronan specific alxM Target Gene: alginate lyase, D-mannuronan specific |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI/SstI--4.1, 2.7; EcoRI/HindIII--2.7 (doublet), 0.6, 0.45, 0.3, 0.25; KpnI--5.1, 1.7; SalI--3.3, (doublet), 0.25. The insert contains the following restriction sites (approximate kb from the 5 end): AccI--0.95; AvaI--1.0, 2.8; KpnI--1.6; SalI--0.95. A periplasmic enzyme preparation of transformed E. coli cells yields 1.7 units per ml of culture broth, compared with 0.4 units per ml of culture broth from the parent organism. The source strain is a gram-negative marine bacterium associated with the brown algae Sargassum fluitans. A 1.6 kb KpnI fragment detects the following genomic restriction fragments in the bacterial strain SFFB080483Alg-A (approximate sizes in kb): EcoRI--23; KpnI--1.8; HincII--2 fragments smaller than 1.8. |
| References： | Brown BJ, et al. Cloning of alginate lyase gene (alxM) and expression in Escherichia coli. Appl. Environ. Microbiol. 57: 1870-1872, 1991. PubMed: 1872617 |
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| 产品名称： | pAL-A3 |
|---|---|
| 商品货号： | TS140998 |
| Designations： | pAL-A3 |
| Species： | Unidentified bacterium |
| Depositors： | BJ Brown |
| Applications： | produces protein alginate lyase, D-mannuronan specific |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.099999904632568 Gene product: alginate lyase, D-mannuronan specific alxM Target Gene: alginate lyase, D-mannuronan specific |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI/SstI--4.1, 2.7; EcoRI/HindIII--2.7 (doublet), 0.6, 0.45, 0.3, 0.25; KpnI--5.1, 1.7; SalI--3.3, (doublet), 0.25. The insert contains the following restriction sites (approximate kb from the 5 end): AccI--0.95; AvaI--1.0, 2.8; KpnI--1.6; SalI--0.95. A periplasmic enzyme preparation of transformed E. coli cells yields 1.7 units per ml of culture broth, compared with 0.4 units per ml of culture broth from the parent organism. The source strain is a gram-negative marine bacterium associated with the brown algae Sargassum fluitans. A 1.6 kb KpnI fragment detects the following genomic restriction fragments in the bacterial strain SFFB080483Alg-A (approximate sizes in kb): EcoRI--23; KpnI--1.8; HincII--2 fragments smaller than 1.8. |
| References： | Brown BJ, et al. Cloning of alginate lyase gene (alxM) and expression in Escherichia coli. Appl. Environ. Microbiol. 57: 1870-1872, 1991. PubMed: 1872617 |
