| 产品名称： | pAMS |
|---|---|
| 商品货号： | TS141137 |
| Designations： | pAMS |
| GenBank Number： | AF010170 |
| Species： | Murine leukemia virus |
| Depositors： | CA Wilson, D Miller |
| Applications： | AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. This AM-MLV clone can be used as a reference standard for detection of murine retroviruses. |
| Vector： | Construct size (kb): 11.32800006866455 |
| Insert： | DNA: genomic Insert lengths(kb): 9.03600025177002 Gene product: AM-MLV* |
| Insert Size (kb)： | 9.036 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of xa0E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--6.8, 4,8; SalI--12.0; BamHI--7.8, 3.6. The plasmid contains the following restriction sites (approximate kb from nt 1 of the GenBank record): BamHI--3.82, 4.13, 7.55; BglI--4.51, 5.98, 7.30, 10.46; ClaI--5.57, 8.23; EcoRI--0.00, 6.96; HindIII--5.49, 5.65, 7.45; KpnI--0.63, 3.45, 8.86; NdeI--5.99, 9.27; PstI--1.16, 1.33, 10.58; PvuI--1.01, 2.71, 10.71; SacI--0.56, 3.15, 8.79; SalI--4.30; SmaI--0.62, 5.10, 8.85; XbaI--0.44, 5.92, 8.67; XhoI--2.15, 6.92. AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. It is a fusion of amphotropic virus 4070A pol-env with Mo-MLV gag region. It can infect a wide range of mouse cell types (including BALB/c) as well as human and chicken cells. This AM-MLV clone can be used as a reference standard for detection of murine retroviruses. The Hybrid Amphotropic/Moloney Murine Leukemia Virus, pAMS, was constructed by inserting the 1541bp SalI-ClaI 4070A pol-env containing fragment into homologous region of Mo-MLV using the cloning vector pBR322 as a backbone. |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| References： | Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952 |
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| 产品名称： | pAMS |
|---|---|
| 商品货号： | TS141137 |
| Designations： | pAMS |
| GenBank Number： | AF010170 |
| Species： | Murine leukemia virus |
| Depositors： | CA Wilson, D Miller |
| Applications： | AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. This AM-MLV clone can be used as a reference standard for detection of murine retroviruses. |
| Vector： | Construct size (kb): 11.32800006866455 |
| Insert： | DNA: genomic Insert lengths(kb): 9.03600025177002 Gene product: AM-MLV* |
| Insert Size (kb)： | 9.036 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of xa0E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--6.8, 4,8; SalI--12.0; BamHI--7.8, 3.6. The plasmid contains the following restriction sites (approximate kb from nt 1 of the GenBank record): BamHI--3.82, 4.13, 7.55; BglI--4.51, 5.98, 7.30, 10.46; ClaI--5.57, 8.23; EcoRI--0.00, 6.96; HindIII--5.49, 5.65, 7.45; KpnI--0.63, 3.45, 8.86; NdeI--5.99, 9.27; PstI--1.16, 1.33, 10.58; PvuI--1.01, 2.71, 10.71; SacI--0.56, 3.15, 8.79; SalI--4.30; SmaI--0.62, 5.10, 8.85; XbaI--0.44, 5.92, 8.67; XhoI--2.15, 6.92. AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. It is a fusion of amphotropic virus 4070A pol-env with Mo-MLV gag region. It can infect a wide range of mouse cell types (including BALB/c) as well as human and chicken cells. This AM-MLV clone can be used as a reference standard for detection of murine retroviruses. The Hybrid Amphotropic/Moloney Murine Leukemia Virus, pAMS, was constructed by inserting the 1541bp SalI-ClaI 4070A pol-env containing fragment into homologous region of Mo-MLV using the cloning vector pBR322 as a backbone. |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| References： | Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952 |
