| 产品名称： | Gallid herpesvirus 2 |
|---|---|
| 商品货号： | TS141459 |
| Classification： | Herpesviridae, Mardivirus |
| Deposited As： | Mareks disease virus type 1 |
| Agent： | Gallid herpesvirus 2 |
| Strain： | JM |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： |  |
| Comments： | Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens. Stable for at least 30 years in viable cells frozen at -196°C. Antigens detectable by immunodiffusion and immunofluorescence.xa0 Antigenically related to herpesvirus of turkeys (HVT), but distinct from infectious laryngotracheitis and duck enteritis virus. Proportion of viral DNA composed of guanine and cytosine is 56-57%. |
| Effect on Host： | CPE, cell rounding and enlargement with some syncytia |
| Recommended Host： |
Duck embryo cells (ATCC® CCL-141™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed by adding infected cells to freshly dissociated cells and planting simultaneously. Incubation: 6-14 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | RL Witter |
| Special Collection： | ATCC |
| Source： | Chicken tumor; blood |
| References： | Sevoian M, Chamberlain DM, and Counter, FT. Avian lymphomatosis. 1. Experimental reproduction of the neural and visceral forms. Vet Med 57: 500-501, 1962. Solomon JJ, et al. Studies on the etiology of Mareks disease. I. Propagation of the agent in cell culture. Proc. Soc. Exp. Biol. Med. 127: 173-177, 1968. PubMed: 5644639 Stephens EA, et al. Characteristics of JMV Mareks disease tumor: a nonproductively infected transplantable cell lacking in rescuable Virus. J. Natl. Cancer Inst. 57: 865-874, 1976. PubMed: 187779 |
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| 产品名称： | Gallid herpesvirus 2 |
|---|---|
| 商品货号： | TS141459 |
| Classification： | Herpesviridae, Mardivirus |
| Deposited As： | Mareks disease virus type 1 |
| Agent： | Gallid herpesvirus 2 |
| Strain： | JM |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： | |
| Comments： | Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens. Stable for at least 30 years in viable cells frozen at -196°C. Antigens detectable by immunodiffusion and immunofluorescence.xa0 Antigenically related to herpesvirus of turkeys (HVT), but distinct from infectious laryngotracheitis and duck enteritis virus. Proportion of viral DNA composed of guanine and cytosine is 56-57%. |
| Effect on Host： | CPE, cell rounding and enlargement with some syncytia |
| Recommended Host： |
Duck embryo cells (ATCC® CCL-141™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed by adding infected cells to freshly dissociated cells and planting simultaneously. Incubation: 6-14 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | RL Witter |
| Special Collection： | ATCC |
| Source： | Chicken tumor; blood |
| References： | Sevoian M, Chamberlain DM, and Counter, FT. Avian lymphomatosis. 1. Experimental reproduction of the neural and visceral forms. Vet Med 57: 500-501, 1962. Solomon JJ, et al. Studies on the etiology of Mareks disease. I. Propagation of the agent in cell culture. Proc. Soc. Exp. Biol. Med. 127: 173-177, 1968. PubMed: 5644639 Stephens EA, et al. Characteristics of JMV Mareks disease tumor: a nonproductively infected transplantable cell lacking in rescuable Virus. J. Natl. Cancer Inst. 57: 865-874, 1976. PubMed: 187779 |
