YpGX265GAL4 [GX4011]
YpGX265GAL4 [GX4011]
规格:
货期:
编号:TS141470
品牌:Testobio
| 产品名称： | YpGX265GAL4 GX4011 |
|---|---|
| 商品货号： | TS141470 |
| Designations： | YpGX265GAL4 GX4011 |
| Depositors： | Genex Corp., SL Strausberg, Genex Corp. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.3000001907348600 Vector: YpGX265GAL4 (plasmid) Promoters: Promoter GAL1-10/MFalpha1 Construction: pJDB207, GAL4, control sequences: GAL1-10, MFalpha1, PHO5, GAPDH Marker(s):ampR,LEU2-d Construct size (kb): 12.30000019073486 Features: marker(s): ampR, LEU2-d promoter: GAL1-10/MFalpha1 replicon: pMB1, 2 micron terminator: GAPDH |
| Applications： | YX-type (expression) shuttle vector expression vector shuttle vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--5.1, 4.2, 3.0; BamHI--8.1, 4.2; HindIII--8.5, 3.8. The plasmid is maintained at approximately 200 copies per cell in haploid S. cerevisiae cells. LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine). High copy number YE-type shuttle expression vector. Note that the XhoI, EcoRV and BamHI sites are not unique in this plasmid. The promoter consists of nt -610 to -157 of the GAL 1-10 upstream activation site, fused to the transcription initiation site (nt -158 to -1) of the alpha mating factor gene MFalpha1. The 3.65 kb HindIII fragment encodes the positive regulator protein GAL4, necessary for transcription from the GAL1-10 promoter. The PHO5 signal sequence (51 nt) was modified to use preferred Saccharomyces codons. The order of the major features in the plasmid is: HindIII - GAL4 - HindIII - Gal1-10 UAS - MFalpha1 transcription initiation sequence - PHO5 signal sequence - EcoRV - BamHI - GAPDH terminator - ampR - LEU2-d - 2 micron ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 39.0°C |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Strausberg RL, Strausberg SL. Composite yeast vectors. US Patent 5,013,652 dated May 7 1991 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | YpGX265GAL4 GX4011 |
|---|---|
| 商品货号： | TS141470 |
| Designations： | YpGX265GAL4 GX4011 |
| Depositors： | Genex Corp., SL Strausberg, Genex Corp. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.3000001907348600 Vector: YpGX265GAL4 (plasmid) Promoters: Promoter GAL1-10/MFalpha1 Construction: pJDB207, GAL4, control sequences: GAL1-10, MFalpha1, PHO5, GAPDH Marker(s):ampR,LEU2-d Construct size (kb): 12.30000019073486 Features: marker(s): ampR, LEU2-d promoter: GAL1-10/MFalpha1 replicon: pMB1, 2 micron terminator: GAPDH |
| Applications： | YX-type (expression) shuttle vector expression vector shuttle vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--5.1, 4.2, 3.0; BamHI--8.1, 4.2; HindIII--8.5, 3.8. The plasmid is maintained at approximately 200 copies per cell in haploid S. cerevisiae cells. LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine). High copy number YE-type shuttle expression vector. Note that the XhoI, EcoRV and BamHI sites are not unique in this plasmid. The promoter consists of nt -610 to -157 of the GAL 1-10 upstream activation site, fused to the transcription initiation site (nt -158 to -1) of the alpha mating factor gene MFalpha1. The 3.65 kb HindIII fragment encodes the positive regulator protein GAL4, necessary for transcription from the GAL1-10 promoter. The PHO5 signal sequence (51 nt) was modified to use preferred Saccharomyces codons. The order of the major features in the plasmid is: HindIII - GAL4 - HindIII - Gal1-10 UAS - MFalpha1 transcription initiation sequence - PHO5 signal sequence - EcoRV - BamHI - GAPDH terminator - ampR - LEU2-d - 2 micron ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 39.0°C |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Strausberg RL, Strausberg SL. Composite yeast vectors. US Patent 5,013,652 dated May 7 1991 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
