| 产品名称： | SYN A4-1 |
|---|---|
| 商品货号： | TS141997 |
| Designations： | SYN A4-1 |
| Species： | Bacillus thuringiensis subsp. israelensis de Barjac |
| Depositors： | Syntro Corporation |
| Vector： | Construct size (kb): 41.0 |
| Insert： | DNA: genomic Insert lengths(kb): 11.0 Gene product: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 11.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | The insert contains the following restriction sites (in approximate kb from the end upstream of the gene): EcoRI--2.5, HpaI--4.0; PstI--6.7; BglII--7.3; HindIII--8.0; EcoRI--10.0. Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 9.0, 3.5, 3.0, 2.3; BamHI-->23.0, 11.0; HindIII-->23.0, 9.0, 4.8, 3.1. The central EcoRI fragment of this construct, and a HpaI/EcoRI fragment derived from that, contain the sequences encoding the complete polypeptide. The insert contains 2 open reading frames. Sequences up to a BalI site at 5.5 kb from the upstream region and after a StuI site in the second reading frame can be deleted without destroying the toxicity of the product to mosquito larvae. |
| References： | Walfield AM, Pollock TJ. Methods and compositions for expression of BTI endotoxin. US Patent 4,652,628 dated Mar 24 1987 Thorne L, et al. Structural similarity between the Lepidoptera- and Diptera-specific insecticidal endotoxin genes of thuringiensis subsp. J. Bacteriol. 166: 801-811, 1986. PubMed: 3011746 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | SYN A4-1 |
|---|---|
| 商品货号： | TS141997 |
| Designations： | SYN A4-1 |
| Species： | Bacillus thuringiensis subsp. israelensis de Barjac |
| Depositors： | Syntro Corporation |
| Vector： | Construct size (kb): 41.0 |
| Insert： | DNA: genomic Insert lengths(kb): 11.0 Gene product: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 11.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | The insert contains the following restriction sites (in approximate kb from the end upstream of the gene): EcoRI--2.5, HpaI--4.0; PstI--6.7; BglII--7.3; HindIII--8.0; EcoRI--10.0. Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 9.0, 3.5, 3.0, 2.3; BamHI-->23.0, 11.0; HindIII-->23.0, 9.0, 4.8, 3.1. The central EcoRI fragment of this construct, and a HpaI/EcoRI fragment derived from that, contain the sequences encoding the complete polypeptide. The insert contains 2 open reading frames. Sequences up to a BalI site at 5.5 kb from the upstream region and after a StuI site in the second reading frame can be deleted without destroying the toxicity of the product to mosquito larvae. |
| References： | Walfield AM, Pollock TJ. Methods and compositions for expression of BTI endotoxin. US Patent 4,652,628 dated Mar 24 1987 Thorne L, et al. Structural similarity between the Lepidoptera- and Diptera-specific insecticidal endotoxin genes of thuringiensis subsp. J. Bacteriol. 166: 801-811, 1986. PubMed: 3011746 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
