pVEX2211 [EKA605]
pVEX2211 [EKA605]
规格:
货期:
编号:TS142029
品牌:Testobio
| 产品名称： | pVEX2211 EKA605 |
|---|---|
| 商品货号： | TS142029 |
| Designations： | pVEX2211 EKA605 |
| Depositors： | DH Figurski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli AS11 |
| Vector Information： | Size (kb): 2.5999999046325680 DESCRIPTION OF VECTOR: Intact vector size: 2.600 Type of vector: plasmid Cloning sites: Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI SacI XhoI BglI HindIII Construction: pVEX2111 Host range: Escherichia coli Features (with orientation and position when available): marker(s): cmLR replicon: R6K Vector: pVEX2211 (plasmid) Construction: pVEX2111 Marker(s):cmlR Construct size (kb): 2.599999904632568 Features: marker(s): cmlR replicon: R6K |
| Applications： | host modification vector for other uses vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.75; HindIII--2.05, 0.65; PvuII--1.5, 1.2. To be used with pVEX1211 or pVEX1212 (ATCC 77420, 77421). The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when chloramphenicol selection is imposed in a host not expressing pir protein. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates pVEX1211(pVEX1212) and pVEX2211(pVEX2212) provide two loxP sites for site-specific recombination that deletes the intervening target sequence. Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. Differs from pVEX2212 (ATCC 77423) only in the orientation of the polylinker. The order of the major features in the plasmid is: R6K ori - loxP - cmlR - NotI - HindIII/MCS/EcoRI - NotI - HindIII. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pVEX2211 EKA605 |
|---|---|
| 商品货号： | TS142029 |
| Designations： | pVEX2211 EKA605 |
| Depositors： | DH Figurski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli AS11 |
| Vector Information： | Size (kb): 2.5999999046325680 DESCRIPTION OF VECTOR: Intact vector size: 2.600 Type of vector: plasmid Cloning sites: Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI SacI XhoI BglI HindIII Construction: pVEX2111 Host range: Escherichia coli Features (with orientation and position when available): marker(s): cmLR replicon: R6K Vector: pVEX2211 (plasmid) Construction: pVEX2111 Marker(s):cmlR Construct size (kb): 2.599999904632568 Features: marker(s): cmlR replicon: R6K |
| Applications： | host modification vector for other uses vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.75; HindIII--2.05, 0.65; PvuII--1.5, 1.2. To be used with pVEX1211 or pVEX1212 (ATCC 77420, 77421). The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when chloramphenicol selection is imposed in a host not expressing pir protein. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates pVEX1211(pVEX1212) and pVEX2211(pVEX2212) provide two loxP sites for site-specific recombination that deletes the intervening target sequence. Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. Differs from pVEX2212 (ATCC 77423) only in the orientation of the polylinker. The order of the major features in the plasmid is: R6K ori - loxP - cmlR - NotI - HindIII/MCS/EcoRI - NotI - HindIII. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
