| 产品名称： | mMT-1 |
|---|---|
| 商品货号： | TS142768 |
| Designations： | mMT-1 |
| GenBank Number： | V00835 |
| Species： | Mus musculus, mouse |
| Depositors： | RD Palmiter |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.850000023841858 Tissue: embryo Gene product: metallothionein 1A (functional)(metallothionein 1, metallothionein 1A) Mt1 Alleles: b, a |
| Insert Size (kb)： | 1.850 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglI/BglII--2.2, 2.0, 1.4, 0.3 (doublet); EcoRI/HindIII--4.4, 1.9; KpnI--6.4; PstI--4.4, 1.9. The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.1; BstEII--0.4; BglI--0.57; BglII--0.82; PstI--1.1. Please note that the promoter is the subject of issued U.S. Patent No. 4,579,821. Corresponds to sequences from -750 to +1100 where +1 is the transcription start point. The region between the BglI site (-185 or nt 116 of the published sequence) and the BglII site (+65 or nt 369 of the published sequence) contains the entire metal-responsive promoter. The vector is a high copy number derivative of pBR322 with a XhoI linker at about 3000 on the standard map). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Durnam DM, et al. Isolation and characterization of the mouse metallothionein-I gene. Proc. Natl. Acad. Sci. USA 77: 6511-6515, 1980. PubMed: 6935664 Glanville N, et al. Structure of mouse metallothionein-I gene and its mRNA. Nature 292: 267-269, 1981. PubMed: 7254320 Palmiter RD, Brinster RL. Control of DNA sequence transcription. US Patent 4,579,821 dated Apr 1 1986 Scherer G, et al. Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5: 275-282, 1989. PubMed: 2571576 Fletcher C, et al. A multilocus linkage map of mouse chromosome 8. Genomics 9: 737-741, 1991. PubMed: 2037298 Richard D Palmiter, personal communication |
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| 产品名称： | mMT-1 |
|---|---|
| 商品货号： | TS142768 |
| Designations： | mMT-1 |
| GenBank Number： | V00835 |
| Species： | Mus musculus, mouse |
| Depositors： | RD Palmiter |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.850000023841858 Tissue: embryo Gene product: metallothionein 1A (functional)(metallothionein 1, metallothionein 1A) Mt1 Alleles: b, a |
| Insert Size (kb)： | 1.850 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglI/BglII--2.2, 2.0, 1.4, 0.3 (doublet); EcoRI/HindIII--4.4, 1.9; KpnI--6.4; PstI--4.4, 1.9. The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.1; BstEII--0.4; BglI--0.57; BglII--0.82; PstI--1.1. Please note that the promoter is the subject of issued U.S. Patent No. 4,579,821. Corresponds to sequences from -750 to +1100 where +1 is the transcription start point. The region between the BglI site (-185 or nt 116 of the published sequence) and the BglII site (+65 or nt 369 of the published sequence) contains the entire metal-responsive promoter. The vector is a high copy number derivative of pBR322 with a XhoI linker at about 3000 on the standard map). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Durnam DM, et al. Isolation and characterization of the mouse metallothionein-I gene. Proc. Natl. Acad. Sci. USA 77: 6511-6515, 1980. PubMed: 6935664 Glanville N, et al. Structure of mouse metallothionein-I gene and its mRNA. Nature 292: 267-269, 1981. PubMed: 7254320 Palmiter RD, Brinster RL. Control of DNA sequence transcription. US Patent 4,579,821 dated Apr 1 1986 Scherer G, et al. Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5: 275-282, 1989. PubMed: 2571576 Fletcher C, et al. A multilocus linkage map of mouse chromosome 8. Genomics 9: 737-741, 1991. PubMed: 2037298 Richard D Palmiter, personal communication |
