| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS142811 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts57 (Dales isolate 7526) |
| Applications： | The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14. |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2 CDC BMBL recommends vaccination. ref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category H indicating that it is a composite of groups D, E and F, with foci of viroplasm, DNA paracrystals, and abberant membranes with and without spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14. |
| Effect on Host： | Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host： | Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions： | Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection not 100% confluent. |
| Name of Depositor： | S Dales, RC Condit, S Dales |
| Special Collection： | NCRR Contract |
| Source： | derived from existing strain |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 |
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| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS142811 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts57 (Dales isolate 7526) |
| Applications： | The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14. |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2 CDC BMBL recommends vaccination. ref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category H indicating that it is a composite of groups D, E and F, with foci of viroplasm, DNA paracrystals, and abberant membranes with and without spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14. |
| Effect on Host： | Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host： | Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions： | Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection not 100% confluent. |
| Name of Depositor： | S Dales, RC Condit, S Dales |
| Special Collection： | NCRR Contract |
| Source： | derived from existing strain |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 |
