| 产品名称： | Human herpesvirus 2 |
|---|---|
| 商品货号： | TS142936 |
| Classification： | Herpesviridae, Simplexvirus |
| Deposited As： | Herpes simplex type 2 |
| Agent： | Human herpesvirus 2 |
| Strain： | G |
| Common Name： | Herpes simplex 2 |
| Applications： | Quality control |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | Deposited as Herpes simplex type 2 Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus. |
| Effect on Host： | CPE, refractile rounding, clumping, detachment |
| Recommended Host： | Vero cells (ATCC CCL-81) |
| Growth Conditions： | Temperature: 34°C
Recommendations for Infection: Plate cells 24 to 48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium.
Incubation: 1 to 5 days in a humidified CO2 atmosphere |
| Name of Depositor： | B Roizman, Institut Merieux |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Source： | Human genital infection |
| References： | Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104 Roizman B, et al. Herpes simplex viruses I and II: a biochemical definition. Perspect. Virol. 8: 129-169, 1973. |
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| 产品名称： | Human herpesvirus 2 |
|---|---|
| 商品货号： | TS142936 |
| Classification： | Herpesviridae, Simplexvirus |
| Deposited As： | Herpes simplex type 2 |
| Agent： | Human herpesvirus 2 |
| Strain： | G |
| Common Name： | Herpes simplex 2 |
| Applications： | Quality control |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： | |
| Comments： | Deposited as Herpes simplex type 2 Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus. |
| Effect on Host： | CPE, refractile rounding, clumping, detachment |
| Recommended Host： | Vero cells (ATCC CCL-81) |
| Growth Conditions： | Temperature: 34°C
Recommendations for Infection: Plate cells 24 to 48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium.
Incubation: 1 to 5 days in a humidified CO2 atmosphere |
| Name of Depositor： | B Roizman, Institut Merieux |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Source： | Human genital infection |
| References： | Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104 Roizman B, et al. Herpes simplex viruses I and II: a biochemical definition. Perspect. Virol. 8: 129-169, 1973. |
