| 产品名称： | Plasmodium falciparum Welch |
|---|---|
| 商品货号： | TS143285 |
| Strain Designations： | FCR-3/Gambia Clone D-3, Knobless |
| Application： | Emerging infectious disease research Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain Isolation date: 1981 |
| Product Format： | frozen |
| Type Strain： | no |
| Antibiotic Resistance： | resistant to chloroquine |
| Comments： | Knobless mutant. |
| Medium： | ATCC® Medium 2196: Malaria medium, complete |
| Growth Conditions： | Temperature: 37.0°C Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol. |
| Cryopreservation： | CRYOPRESERVATION:xa0 xa0xa0xa0xa0xa0xa0xa0 Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust. 1.xa0xa0xa0xa0 Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube. 2.xa0xa0xa0xa0 Aspirate supernatant using sterile Pasteur pipet. 3.xa0xa0xa0xa0 Resuspend pellet gently in remaining supernatant. 4.xa0xa0xa0xa0 Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows: xa0xa0xa0xa0xa0xa0xa0 A.xa0xa0xa0 Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature. B.xa0xa0xa0 Add the remaining 4 volumes of glycerolyte and gently agitate. 5.xa0xa0xa0xa0 Aliquot mixture into Nunc screwtop freezing vials and store at x9680oC overnight. 6.xa0xa0xa0xa0 Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor. ** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:xa0 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4oC. |
| Name of Depositor： | W Trager |
| Year of Origin： | 1981 |
| References： | Trager W, et al. Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes. Proc. Natl. Acad. Sci. USA 78: 6527-6530, 1981. PubMed: 7031656 |
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| 产品名称： | Plasmodium falciparum Welch |
|---|---|
| 商品货号： | TS143285 |
| Strain Designations： | FCR-3/Gambia Clone D-3, Knobless |
| Application： | Emerging infectious disease research Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain Isolation date: 1981 |
| Product Format： | frozen |
| Type Strain： | no |
| Antibiotic Resistance： | resistant to chloroquine |
| Comments： | Knobless mutant. |
| Medium： | ATCC® Medium 2196: Malaria medium, complete |
| Growth Conditions： | Temperature: 37.0°C Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol. |
| Cryopreservation： | CRYOPRESERVATION:xa0 xa0xa0xa0xa0xa0xa0xa0 Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust. 1.xa0xa0xa0xa0 Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube. 2.xa0xa0xa0xa0 Aspirate supernatant using sterile Pasteur pipet. 3.xa0xa0xa0xa0 Resuspend pellet gently in remaining supernatant. 4.xa0xa0xa0xa0 Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows: xa0xa0xa0xa0xa0xa0xa0 A.xa0xa0xa0 Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature. B.xa0xa0xa0 Add the remaining 4 volumes of glycerolyte and gently agitate. 5.xa0xa0xa0xa0 Aliquot mixture into Nunc screwtop freezing vials and store at x9680oC overnight. 6.xa0xa0xa0xa0 Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor. ** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:xa0 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4oC. |
| Name of Depositor： | W Trager |
| Year of Origin： | 1981 |
| References： | Trager W, et al. Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes. Proc. Natl. Acad. Sci. USA 78: 6527-6530, 1981. PubMed: 7031656 |
