LPL-35-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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LPL-35
LPL-35
规格:
货期:
编号:TS143632
品牌:Testobio
产品名称: LPL-35
商品货号: TS143632
Designations: LPL-35
GenBank Number:

M15856

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-2
Intact vector size: 2.870
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->, 10-60
promoter for in vitro transcription: T7, replicon: pMB1
marker(s): ampR, promoter for in vitro transcription: SP6, ->, 2852-2869
Cross references: DNA Seq. Acc.: X65301
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LPL
Other gene symbols: LIPD
Genomic copy number: unique
Gene name: lipoprotein lipase
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p22
Tissue: adipose
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: SalI linker
Insert size (kb): 2.435
Cross references: DNA Seq. Acc.: M15856
Nucleotides ~1-2425 of the insert correspond to
nucleotides 1-2425 of M15856.
Insert lengths(kb): 2.434999942779541
Tissue: adipose
Gene product: lipoprotein lipase LPL
Alleles: Ca1, Ca2, A1, A2, A3, Ca1, Ca2, Da1, A1, A2, A3, Da1, Da2, Da2
Insert Size (kb): 2.435
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.5; EcoRI--4.5, 0.5, 0.35; XbaI--5.4; HindIII--5.4; PstI--5.4.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.64, 1.98; XbaI--2.26; PvuII--0.32, 0.73, 2.39.
References:

Wion KL, et al. Human lipoprotein lipase complementary DNA sequence. Science 235: 1638-1641, 1987. PubMed: 3823907

Michael C Schotz, personal communication

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LPL-35

  • 货号: TS143632
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: LPL-35
商品货号: TS143632
Designations: LPL-35
GenBank Number:

M15856

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-2
Intact vector size: 2.870
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->, 10-60
promoter for in vitro transcription: T7, replicon: pMB1
marker(s): ampR, promoter for in vitro transcription: SP6, ->, 2852-2869
Cross references: DNA Seq. Acc.: X65301
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LPL
Other gene symbols: LIPD
Genomic copy number: unique
Gene name: lipoprotein lipase
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p22
Tissue: adipose
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: SalI linker
Insert size (kb): 2.435
Cross references: DNA Seq. Acc.: M15856
Nucleotides ~1-2425 of the insert correspond to
nucleotides 1-2425 of M15856.
Insert lengths(kb): 2.434999942779541
Tissue: adipose
Gene product: lipoprotein lipase LPL
Alleles: Ca1, Ca2, A1, A2, A3, Ca1, Ca2, Da1, A1, A2, A3, Da1, Da2, Da2
Insert Size (kb): 2.435
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.5; EcoRI--4.5, 0.5, 0.35; XbaI--5.4; HindIII--5.4; PstI--5.4.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.64, 1.98; XbaI--2.26; PvuII--0.32, 0.73, 2.39.
References:

Wion KL, et al. Human lipoprotein lipase complementary DNA sequence. Science 235: 1638-1641, 1987. PubMed: 3823907

Michael C Schotz, personal communication

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