S-ENDO3.3-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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S-ENDO3.3
S-ENDO3.3
规格:
货期:
编号:TS144053
品牌:Testobio
产品名称: S-ENDO3.3
商品货号: TS144053
Designations: S-ENDO3.3
GenBank Number:

X72012

Species: Homo sapiens, human
Depositors: C Bernabeu
Vector:
Construct size (kb): 5.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC13
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Construction: pBR322, Escherichia coli lacZalpha, M13mp11
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ENG
Other gene symbols: HHT1; ORW1; END
Gene name: endoglin (Osler-Rendu-Weber syndrome 1)
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: HL-60 cell line, PMA-treated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.1
Cross references: DNA Seq. Acc.: X72012
Nucleotides 1-3073 of the insert correspond to
nucleotides 1-3073 of X72012.
Insert lengths(kb): 3.099999904632568
Tissue: HL-60 cell line, PMA-treated
Gene product: endoglin (Osler-Rendu-Weber syndrome 1)(Endoglin) END
Insert Size (kb): 3.100
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; HindIII--5.9; BamHI/MluI--3.0, 2.3, 0.58; BamHI--3.0, 2.9; PstI--4.4, 0.87, 0.68.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.7; BglI--3.0; PstI--0.33, 1.04, 1.28.
Encodes the S-isoform with a monomeric molecular weight of 85 kDa. This form, which is not the predominant one, includes an exon of 135 bp.
References:

Bellon T, et al. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23: 2340-2345, 1993. PubMed: 8370410

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S-ENDO3.3

  • 货号: TS144053
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: S-ENDO3.3
商品货号: TS144053
Designations: S-ENDO3.3
GenBank Number:

X72012

Species: Homo sapiens, human
Depositors: C Bernabeu
Vector:
Construct size (kb): 5.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC13
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Construction: pBR322, Escherichia coli lacZalpha, M13mp11
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ENG
Other gene symbols: HHT1; ORW1; END
Gene name: endoglin (Osler-Rendu-Weber syndrome 1)
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: HL-60 cell line, PMA-treated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.1
Cross references: DNA Seq. Acc.: X72012
Nucleotides 1-3073 of the insert correspond to
nucleotides 1-3073 of X72012.
Insert lengths(kb): 3.099999904632568
Tissue: HL-60 cell line, PMA-treated
Gene product: endoglin (Osler-Rendu-Weber syndrome 1)(Endoglin) END
Insert Size (kb): 3.100
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; HindIII--5.9; BamHI/MluI--3.0, 2.3, 0.58; BamHI--3.0, 2.9; PstI--4.4, 0.87, 0.68.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.7; BglI--3.0; PstI--0.33, 1.04, 1.28.
Encodes the S-isoform with a monomeric molecular weight of 85 kDa. This form, which is not the predominant one, includes an exon of 135 bp.
References:

Bellon T, et al. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23: 2340-2345, 1993. PubMed: 8370410

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