| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS144445 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | MW3271-10C pms1-delta |
| Alternate State： | Candida robusta Diddens et Lodder |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa pms1-delta leu2-3 leu2-112 lys2-20 his4-519 ade8-deltaKpn ura3-52 hom3-10 thr1 lys1-1 ade2 arg4-deltaSst1
Ref  Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, RefKramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.) |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | Meiosis-sporulation: postmeiotic segregation increase (CL2) |
| Medium： | ATCC® Medium 1069: YPAD medium |
| Growth Conditions： | Temperature: 25.0°C Atmosphere: Typical aerobic Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Subcultivation： | Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Name of Depositor： | YGSC |
| Special Collection： | Yeast Genetic Stock Center |
| Chain of Custody： | ATCC < |
| References： | Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926 Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974 Isogenic with MW3271-10C (ATCC 208348) except for the pms locus. Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926 Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974 Isogenic with MW3271-10C (ATCC 208348) except for the pms locus. |
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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS144445 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | MW3271-10C pms1-delta |
| Alternate State： | Candida robusta Diddens et Lodder |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa pms1-delta leu2-3 leu2-112 lys2-20 his4-519 ade8-deltaKpn ura3-52 hom3-10 thr1 lys1-1 ade2 arg4-deltaSst1
RefWilliamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, RefKramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.) |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | Meiosis-sporulation: postmeiotic segregation increase (CL2) |
| Medium： | ATCC® Medium 1069: YPAD medium |
| Growth Conditions： | Temperature: 25.0°C Atmosphere: Typical aerobic Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Subcultivation： | Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
| Name of Depositor： | YGSC |
| Special Collection： | Yeast Genetic Stock Center |
| Chain of Custody： | ATCC < |
| References： | Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926 Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974 Isogenic with MW3271-10C (ATCC 208348) except for the pms locus. Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926 Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974 Isogenic with MW3271-10C (ATCC 208348) except for the pms locus. |
