| 产品名称： | pH15C |
|---|---|
| 商品货号： | TS144750 |
| Designations： | pH15C |
| GenBank Number： | M57627 |
| Species： | Homo sapiens, human |
| Depositors： | DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications： | in another host, produces protein interleukin 10 |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: T-lymphocyte B21 cell line, activated Gene product: interleukin 10 IL10 Target Gene: interleukin 10 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 1.9; HindIII--3.7, 1.8; BglII/HindIII--3.7, 1.1, 0.7; PvuII--4.4, 1.1. The insert contains the following restriction sites (approximate kb from the 5 end): PvuI--0.15, 0.21; HindIII--0.92; EcoRV--1.41. Contains the complete coding sequence (nt 31-567). The mobility of the protein produced by COS-7 transfectants is not affected by tunicamycin. The 3 untranslated region contains an Alu repeat. A 0.7 kb BglII/HindIII fragment from the insert is repeat-free. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| References： | Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993 Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pH15C |
|---|---|
| 商品货号： | TS144750 |
| Designations： | pH15C |
| GenBank Number： | M57627 |
| Species： | Homo sapiens, human |
| Depositors： | DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications： | in another host, produces protein interleukin 10 |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: T-lymphocyte B21 cell line, activated Gene product: interleukin 10 IL10 Target Gene: interleukin 10 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 1.9; HindIII--3.7, 1.8; BglII/HindIII--3.7, 1.1, 0.7; PvuII--4.4, 1.1. The insert contains the following restriction sites (approximate kb from the 5 end): PvuI--0.15, 0.21; HindIII--0.92; EcoRV--1.41. Contains the complete coding sequence (nt 31-567). The mobility of the protein produced by COS-7 transfectants is not affected by tunicamycin. The 3 untranslated region contains an Alu repeat. A 0.7 kb BglII/HindIII fragment from the insert is repeat-free. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| References： | Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993 Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
