| 产品名称： | pMCV1-E-D+I purified plasmid DNA |
|---|---|
| 商品货号： | TS145035 |
| Designations： | pMCV1-E-D+I purified plasmid DNA |
| GenBank Definition： | Molluscum contagiosum virus subtype 1, complete genome |
| GenBank Number： | U60315 |
| Depositors： | J Bugert |
| Vector： | Total size of construct (kb): 29.732 Marker: tetR Vector: pACYC184 Vector type: cloning vector Insert site: EcoRI Vector size (kb): 4.245 Suggested primer for sequencing from the 5’ side of the EcoRI site: Primer yE-1 (forward): 5’-GTCGTGGTATTCACTCCAGAGCGAT-3’ Primer yE-2 (reverse): 5’-GCATTTCAGTCAGTTGCTCAATGTACCTATA-3’ |
| Insert： | Insert size (kb): 25.487 Insert ends: EcoRI DNA: partial genomic DNA Source: Molluscum contagiosum virus (MCV) Genbank accession: U60315, partial Nucleotides 1-25487 of the insert correspond to nucleotides 83880-109367 of Genbank U60315 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed as 200 ng purified plasmid DNA |
| Comments： | This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The insert is 25.487 kb and contains an internal EcoRI site. Sequence of the insert corresponds to nucleotides 83880-109367 of Genbank sequence U60315 for the complete genome of the virus. The internal EcoRI site is at nt 107692. |
| References： | Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497 Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026 Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425 |
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| 产品名称： | pMCV1-E-D+I purified plasmid DNA |
|---|---|
| 商品货号： | TS145035 |
| Designations： | pMCV1-E-D+I purified plasmid DNA |
| GenBank Definition： | Molluscum contagiosum virus subtype 1, complete genome |
| GenBank Number： | U60315 |
| Depositors： | J Bugert |
| Vector： | Total size of construct (kb): 29.732 Marker: tetR Vector: pACYC184 Vector type: cloning vector Insert site: EcoRI Vector size (kb): 4.245 Suggested primer for sequencing from the 5’ side of the EcoRI site: Primer yE-1 (forward): 5’-GTCGTGGTATTCACTCCAGAGCGAT-3’ Primer yE-2 (reverse): 5’-GCATTTCAGTCAGTTGCTCAATGTACCTATA-3’ |
| Insert： | Insert size (kb): 25.487 Insert ends: EcoRI DNA: partial genomic DNA Source: Molluscum contagiosum virus (MCV) Genbank accession: U60315, partial Nucleotides 1-25487 of the insert correspond to nucleotides 83880-109367 of Genbank U60315 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed as 200 ng purified plasmid DNA |
| Comments： | This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The insert is 25.487 kb and contains an internal EcoRI site. Sequence of the insert corresponds to nucleotides 83880-109367 of Genbank sequence U60315 for the complete genome of the virus. The internal EcoRI site is at nt 107692. |
| References： | Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497 Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026 Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425 |
