| 产品名称： | p144-2 |
|---|---|
| 商品货号： | TS145152 |
| Designations： | p144-2 |
| Species： | Homo sapiens, human |
| Depositors： | HG Zachau, W Pargent |
| Applications： | The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic. |
| Vector： | Construct size (kb): 3.349999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.3499999940395355 Tissue: placenta St Gene product: immunoglobulin kappa variable region IGKV Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp |
| Insert Size (kb)： | 0.350 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.5, 0.3; PvuI--2.2, 1.2; PvuI/EcoRI--1.7, 1.1, 0.6; PstI--3.4; PvuII/XbaI--2.5, 0.3, 0.3, 0.2. Detects sequences from the Op (between O12 and O13) and Od (between O2 and O2) regions. Does not cross-hybridize to the W region. The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic. Contains the SalI fragment from m144-2 which had been generated by adding SalI linkers to an RsaI fragment. During recloning, the SalI end 150 bp from the vector PvuII site was destroyed. Neither KpnI, ApaI nor XhoI cut at this position. The insert is not readily excised, but can be amplified with standard M13 sequencing (forward and reverse) primers. |
| References： | Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350 Pohlenz HD, et al. The human V(k) locus: characterization of extended immunoglobulin gene regions by cosmid cloning. J. Mol. Biol. 193: 241-253, 1987. PubMed: 3037085 Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917 Hans G Zachau, personal communication |
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| 产品名称： | p144-2 |
|---|---|
| 商品货号： | TS145152 |
| Designations： | p144-2 |
| Species： | Homo sapiens, human |
| Depositors： | HG Zachau, W Pargent |
| Applications： | The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic. |
| Vector： | Construct size (kb): 3.349999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.3499999940395355 Tissue: placenta St Gene product: immunoglobulin kappa variable region IGKV Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp |
| Insert Size (kb)： | 0.350 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.5, 0.3; PvuI--2.2, 1.2; PvuI/EcoRI--1.7, 1.1, 0.6; PstI--3.4; PvuII/XbaI--2.5, 0.3, 0.3, 0.2. Detects sequences from the Op (between O12 and O13) and Od (between O2 and O2) regions. Does not cross-hybridize to the W region. The EcoRI polymorphism can be used only for haplotype G and the BglII polymorphism can be used only for haplotype N. In the KpnI/BglII and KpnI/SacI digests, the KpnI site is polymorphic. Contains the SalI fragment from m144-2 which had been generated by adding SalI linkers to an RsaI fragment. During recloning, the SalI end 150 bp from the vector PvuII site was destroyed. Neither KpnI, ApaI nor XhoI cut at this position. The insert is not readily excised, but can be amplified with standard M13 sequencing (forward and reverse) primers. |
| References： | Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350 Pohlenz HD, et al. The human V(k) locus: characterization of extended immunoglobulin gene regions by cosmid cloning. J. Mol. Biol. 193: 241-253, 1987. PubMed: 3037085 Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917 Hans G Zachau, personal communication |
