| 产品名称： | GC-2.2 GCS-2kb, lambda-EZZ-gamma3 |
|---|---|
| 商品货号： | TS145181 |
| Designations： | GC-2.2 GCS-2kb, lambda-EZZ-gamma3 |
| GenBank Number： | M11080 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.200000047683716 Tissue: fibroblast WI-38 cell line Gene product: glucosidase, beta; acid(Glucosidase, beta, acid, glucocerebrosidase) GBA Target Gene: glucosidase, beta; acid |
| Insert Size (kb)： | 2.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 1.7, 1.0; EcoRI--4.4, 2.3; HindIII--4.6, 2.1; PvuII--3.7, 3.1; SalI--5.2, 1.6. The insert includes the following restriction sites (kb from the 5 end): BamHI--0.027, 1.629; HindIII--0.231; ScaI--1.022; SalI--1.403. Contains the complete coding sequence but lacks some of the 5 untranslated region. The coding sequences extend from nt 154 to 1709 of the published sequence. Constructed from G5A-IY and ID9bb by taking advantage of the unique ScaI site in the region of overlap. |
| References： | Zimran A, et al. Linkage of the PvuII polymorphism with the common Jewish mutation for gaucher disease. Am. J. Hum. Genet. 46: 902-905, 1990. PubMed: 1971142 Dahl N, et al. Gaucher disease type III (Norrbottnian type) is caused by a single mutation in exon 10 of the glucocerebrosidase gene. Am. J. Hum. Genet. 47: 275-278, 1990. PubMed: 2378352 Sorge J, et al. Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA. Proc. Natl. Acad. Sci. USA 82: 7289-7293, 1985. PubMed: 3864160 Sorge J, et al. Correction. Proc. Natl. Acad. Sci. USA 83: 3567, 1986. Sorge JA, et al. The human glucocerebrosidase gene has two funtional ATC initiator codons. Am. J. Hum. Genet. 41: 1016-1024, 1987. PubMed: 3687939 |
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| 产品名称： | GC-2.2 GCS-2kb, lambda-EZZ-gamma3 |
|---|---|
| 商品货号： | TS145181 |
| Designations： | GC-2.2 GCS-2kb, lambda-EZZ-gamma3 |
| GenBank Number： | M11080 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.200000047683716 Tissue: fibroblast WI-38 cell line Gene product: glucosidase, beta; acid(Glucosidase, beta, acid, glucocerebrosidase) GBA Target Gene: glucosidase, beta; acid |
| Insert Size (kb)： | 2.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 1.7, 1.0; EcoRI--4.4, 2.3; HindIII--4.6, 2.1; PvuII--3.7, 3.1; SalI--5.2, 1.6. The insert includes the following restriction sites (kb from the 5 end): BamHI--0.027, 1.629; HindIII--0.231; ScaI--1.022; SalI--1.403. Contains the complete coding sequence but lacks some of the 5 untranslated region. The coding sequences extend from nt 154 to 1709 of the published sequence. Constructed from G5A-IY and ID9bb by taking advantage of the unique ScaI site in the region of overlap. |
| References： | Zimran A, et al. Linkage of the PvuII polymorphism with the common Jewish mutation for gaucher disease. Am. J. Hum. Genet. 46: 902-905, 1990. PubMed: 1971142 Dahl N, et al. Gaucher disease type III (Norrbottnian type) is caused by a single mutation in exon 10 of the glucocerebrosidase gene. Am. J. Hum. Genet. 47: 275-278, 1990. PubMed: 2378352 Sorge J, et al. Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA. Proc. Natl. Acad. Sci. USA 82: 7289-7293, 1985. PubMed: 3864160 Sorge J, et al. Correction. Proc. Natl. Acad. Sci. USA 83: 3567, 1986. Sorge JA, et al. The human glucocerebrosidase gene has two funtional ATC initiator codons. Am. J. Hum. Genet. 41: 1016-1024, 1987. PubMed: 3687939 |
