pRS412
pRS412
规格:
货期:
编号:TS145413
品牌:Testobio
| 产品名称： | pRS412 |
|---|---|
| 商品货号： | TS145413 |
| Designations： | pRS412 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.042 DESCRIPTION OF VECTOR: Intact vector size: 6.042 Type of vector: phagemid Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI Construction: pRS402, pRS411 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ADE2, ->, 575-2290 replicon: f1, promoter for in vitro transcription: T7, ->, 3121-3140 MCS: KpnI...SacI, ->, 3148-3250 promoter for in vitro transcription: T3, insert detection: lacZ, promoter: lac, replicon: pMB1, 3710-3710 marker(s): ampR, centromere: CEN6, 5470-5586 replicon: ARS4, 5587-5988 Vector: pRS412 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS402, pRS411 Marker(s):ampR,ADE2 Construct size (kb): 6.042 Features: insert detection: lacZ marker(s): ADE2 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--2.2, 3.8; EcoRI--6.0. ade2 phenotype produces red colonies when grown on adenine containing media. This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | component of:ATCC 87480 component of:ATCC 87530 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pRS412 |
|---|---|
| 商品货号： | TS145413 |
| Designations： | pRS412 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.042 DESCRIPTION OF VECTOR: Intact vector size: 6.042 Type of vector: phagemid Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI Construction: pRS402, pRS411 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ADE2, ->, 575-2290 replicon: f1, promoter for in vitro transcription: T7, ->, 3121-3140 MCS: KpnI...SacI, ->, 3148-3250 promoter for in vitro transcription: T3, insert detection: lacZ, promoter: lac, replicon: pMB1, 3710-3710 marker(s): ampR, centromere: CEN6, 5470-5586 replicon: ARS4, 5587-5988 Vector: pRS412 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS402, pRS411 Marker(s):ampR,ADE2 Construct size (kb): 6.042 Features: insert detection: lacZ marker(s): ADE2 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--2.2, 3.8; EcoRI--6.0. ade2 phenotype produces red colonies when grown on adenine containing media. This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | component of:ATCC 87480 component of:ATCC 87530 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
