MKK1a-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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MKK1a
MKK1a
规格:
货期:
编号:TS146073
品牌:Testobio
产品名称: MKK1a
商品货号: TS146073
Designations: MKK1a
GenBank Number:

L05624

Species: Homo sapiens, human
Depositors: EG Krebs
Applications:
in another host, produces protein mitogen-activated protein kinase kinase protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase
Vector:
Construct size (kb): 6.454999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Amp
Intact vector size: 5.000
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
marker(s): ampR
promoter: CMV, T7, SP6
replicon: polyoma, ColE1, M13
terminator: SV40
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKK*
Gene name: mitogen-activated protein kinase kinase
Contains complete coding sequence?: U
Tissue: T-lymphocyte
Type of DNA: cDNA
Insert 5 end: BamHI
Insert 3 end: EcoRI
Insert size (kb): 1.455
Cross references: DNA Seq. Acc.: L05624
Insert lengths(kb): 1.455000042915344
Tissue: T-lymphocyte
Gene product: mitogen-activated protein kinase kinase( protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase) MKK*
Target Gene: mitogen-activated protein kinase kinase
Insert Size (kb): 1.455
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRV--5.0, 1.45; BglII--6.5; EcoRI--5.5, 1.0; PvuII--2.8, 1.95, 1.7; HaeII--3.6, 2.2, 0.7.
The insert contains the following restriction sites (bp from the 5 end): AflIII--719; AlwI--486; BanI--860; BglII--1120; BspMII--353; BsrI--1010; BstUI--15; EcoRI--512; HaeII--176; HgiAI--463; HpaI--91;
HphI--1314; PvuII--61; RsaI--405; SspI--1374; TaqI--680.
A probe from nt 1-516 of the insert detects a 2.6 kb mRNA in human thymus, brain, kidney, muscle, liver, intestine, heart, lung, spleen and EGF-stimulated A431 cells.
References:

Seger R, et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. J. Biol. Chem. 267: 25628-25631, 1992. PubMed: 1281467

Edwin G Krebs, personal communication

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MKK1a

  • 货号: TS146073
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: MKK1a
商品货号: TS146073
Designations: MKK1a
GenBank Number:

L05624

Species: Homo sapiens, human
Depositors: EG Krebs
Applications:
in another host, produces protein mitogen-activated protein kinase kinase protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase
Vector:
Construct size (kb): 6.454999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Amp
Intact vector size: 5.000
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
marker(s): ampR
promoter: CMV, T7, SP6
replicon: polyoma, ColE1, M13
terminator: SV40
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKK*
Gene name: mitogen-activated protein kinase kinase
Contains complete coding sequence?: U
Tissue: T-lymphocyte
Type of DNA: cDNA
Insert 5 end: BamHI
Insert 3 end: EcoRI
Insert size (kb): 1.455
Cross references: DNA Seq. Acc.: L05624
Insert lengths(kb): 1.455000042915344
Tissue: T-lymphocyte
Gene product: mitogen-activated protein kinase kinase( protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase) MKK*
Target Gene: mitogen-activated protein kinase kinase
Insert Size (kb): 1.455
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRV--5.0, 1.45; BglII--6.5; EcoRI--5.5, 1.0; PvuII--2.8, 1.95, 1.7; HaeII--3.6, 2.2, 0.7.
The insert contains the following restriction sites (bp from the 5 end): AflIII--719; AlwI--486; BanI--860; BglII--1120; BspMII--353; BsrI--1010; BstUI--15; EcoRI--512; HaeII--176; HgiAI--463; HpaI--91;
HphI--1314; PvuII--61; RsaI--405; SspI--1374; TaqI--680.
A probe from nt 1-516 of the insert detects a 2.6 kb mRNA in human thymus, brain, kidney, muscle, liver, intestine, heart, lung, spleen and EGF-stimulated A431 cells.
References:

Seger R, et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. J. Biol. Chem. 267: 25628-25631, 1992. PubMed: 1281467

Edwin G Krebs, personal communication

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